458 Mr. A. J. Walton. Growth of Adult Mammalian 



The homogenous plasma gave 100 per cent, positive results and growth 

 was very well marked in it. Only 13 per cent, grew in the autogenous 

 plasma and growth in it was slight. 



Experiment 9. — Thyroid tissue was cultivated — 



(1) In plasma taken from animal 7, now nine days old. 



(2) In the plasma taken from animal 8, now four days old. 



(3) In fresh autogenous plasma. 



In the nine-day plasma 60 per cent, of the cultures grew, the growth being 

 fairly extensive. 



In the four-day plasma 67 per cent, grew, and the growth was very well 

 marked. 



In the fresh autogenous plasma 44 per cent, of the tissues grew, and growth 

 in these was less extensive than in the other groups. 

 Experiment 10. — Thyroid tissue was cultivated — 



(1) In homogenous plasma taken from animal 7, now twelve days old. 



(2) In that taken from animal 8, now seven days old. 



(3) In that taken from animal 9, now three days old. 



(4) In fresh autogenous plasma. 



In the plasmata from animals 7 and 8 there was no growth at all. In the 

 plasma from animal 9 100 per cent, of the pieces grew and the growth of these 

 was very well marked. In the fresh autogenous plasma 44 per cent. grew. 

 The growth of these was much less marked than that of those grown in the. 

 three-day homogenous plasma. 



These results are shown in the following table : — 



Plasma. 



Animal 7. 



Animal 8. 



Animal 9. 



Animal 10. 



7 



Fresh 



5 days, 



9 days. 



12 days, 





42 per cent. 



100 per cent. 



60 per cent. 







8 





Fresh, 



4 days, 



7 days, 







13 per cent. 



67 per cent. 







9 







Fresh, 



3 days, 









44 per cent. 



100 per cent. 



10 









Fresh, 











44 per cent. 



It was clear that the increase in the amount of growth which took place 

 when the plasma had been kept frozen for about three days was very marked, 

 thus while autogenous plasma when fresh gave a growth in from 13 per cent, 

 to 40 per cent, of cases, it gave a growth in 100 per cent, of the trials when 

 it had been kept for three days and was homogenous. The fact that some 

 plasmata are good and others bad is also clearly shown by the table. For 

 instance, the plasma of animal 8 is definitely not so good as that of auimals 



