516 



Sir D. Bruce and others. Trypanosome 



Conclusion. 



The pathogenic action of T. brucei, Zululand strain, 1913, on various 

 animals is so similar, not only in regard to the symptoms during life' 

 but also in the post-mortem appearances and rate of mortality, to that of the 

 trypanosome causing disease in man in Nyasaland, that it affords another 

 proof that these two trypanosomes are identical. 



The Trypanosome causing Disease in Man in Nyasaland. 

 Part III. — Development in Glossina morsitans. 



By Surgeon-General Sir David Bruce, C.B., F.R.S., A.M.S. ; Major A. E. 

 Hamerton, D.S.O., and Captain D. P. Watson, R.A.M.C. ; and 

 Lady Bruce, R.R.C. (Scientific Commission of the Royal Society, 

 Nyasaland, 1912-14.) 



(Received March 17,— Read March 26, 1914.) 

 [Plate 24.] 



Introduction. 



In previous papers* the morphology of this trypanosome and the suscep- 

 tibility of various animals to its pathogenic action have been described. In 

 this is given an account of its development in Glossina morsitans. 



In Uganda the study of the development of Trypanosoma gambiense in 

 G. palpalis was much assisted by the circumstance that large numbers 

 of laboratory-bred tsetse flies were available. This was due to the fact 

 that the pupse of G. palpalis could be collected on the lake-shore in prac- 

 tically unlimited numbers. It is quite otherwise with G. morsitans. It has 

 been found impossible to find the pupae of this species in any numbers, so 

 that all laboratory-bred G. morsitans have had to be hatched out of pupae 

 obtained from captive flies, a slow and laborious process. The flies are 

 caught some 20 to 30 miles from the laboratory and brought up to Kasu camp 

 by a native on a bicycle. This kills a large number of the flies. Moreover, 

 the climatic conditions at the camp are not always favourable for breeding 

 and hatching out. This was remedied to some extent by establishing a 

 breeding station down in the low-country, but as this had to be left in the 

 charge of natives the results were not always very satisfactory. 



* ' Boy. Soc. Proc.,' B, vol. 85 (1912), and B, vols. 86 and 87 (1913). 



