causing Disease in Man in Nyasaland. 



519 



Details of the Eight Positive Experiments. 

 The following table gives the details of the eight positive experiments : — 



Table III. 



Expt. 



Day of expt. 



Procedure. 



Eemarks. 



1003 



1-2 

 3 



4-41 



Flies red on infected dog. 

 Starved. 



Fed on clean Monkey 1023. 



Trypanosomes appeared in 

 blood of Monkey 1023 on 

 the 38th day. 



1 i Zo 



1—4 

 5 



6-22 



rues tea on miectea clog. 

 Starved. 



Fed on clean Monkey 1733. 



Trypanosomes appeared in 

 blood of Monkey 1733 on 

 the 21st day. 





1-6 



7 

 8-32 



riies red on lnlected monkey. 

 Starved. 



Fed on clean Monkey 2410. 



Trypanosomes appeared in 

 blood of Monkey 2410 on 

 the 30th day. 



iooU 



1-2 

 3 



4-22 



Flies fed on infected dog. 

 Starved. 



Fed on clean Dog 1708. 



Trypanosomes appeared in 

 blood of Dog 1708 on the 

 25th day. 



1688 



1-2 

 3 



4-12 



Flies fed on infected monkey. 

 Starved. 



Fed on clean Monkey 1699. 



Trypanosomes appeared in 

 blood of Monkey 1699 on 

 the 10th day. 



1705 



1-2 



3 

 4-9 



Flies fed on infected monkey. 

 Starved. 



Fed on clean Monkey 1707. 



Trypanosomes appeared in 

 blood of Monkey 1707 on 

 the 8th day. 



1748 



1-2 

 3 



4-30 



Flies fed on infected monkey. 

 Starved. 



Fed on clean Monkey 1845. 



Trypanosomes appeared in 

 blood of Monkey 1845 on 

 the 32nd day. 



1729 



1-2 

 3 



4-38 



Flies fed on infected dog. 

 Starved. 



Fed on clean Dog 1767. 



Trypanosomes appeared in 

 blood of Dog 1767 on the 

 37th day. 



Omitting Experiments 1688 and 1705, it would appear from the remaining 

 six experiments that an average period of 24 days is required to complete the 

 cycle of development of the trypanosome causing disease in man in Nyasaland 

 in G. morsitans, the flies being kept at a temperature of 84° F. 



Details of the Eight Negative Experiments. 



The following table shows the method of procedure in carrying out the 

 eight negative experiments : — 



