Notes on the Genus Toxoplasma. 



295 



I am much indebted to Mr. A. T. Watson, the laboratory assistant at the 

 Zoological Gardens, for very intelligent help in the preparation of the 

 specimens, and to Dr. A. Norman for the photographs of a difficult object. 



[Note added May 1, 1916. — Since the above was written I have found 

 Toxoplasmas in another bird, a Pied Bush Chat (Pratincola caprata) from 

 India. They were found in the blood and exudation from the lungs, which 

 were inflamed and cedematous, in the mononuclear leucocytes as before. 

 Further early stages of schizogony were found in the large endothelial cells 

 in the lungs.] 



KEFERENCES. 



1. Splendore, A. "Um novo Protozoo parasita de conigli," 'Rev. da Soc. Scientifica de ■ 



S. Paulo,' Nos. 10-12, 1908. 



2. Nicolle, C, and Manceaux, L. " Sur une infection a, corps de Leishman du Gondi." 



' Compt. Rend.,' October, 1908, and February, 1909. 



3. Fry, W. B., and Ranken, H. S. " The Extrusion of Granules by Trypanosonies," ' Roy. 



Soc. Proc.,' B, vol. 84, p. 79, and vol. 86, p. 380. 



4. Minchin, E. A. " On the So-called ' Infective Granules,' " ' Ann. de l'Institut Pasteur,' 



vol. 29, p. 537. 



5. Pixell, H. L. M. " Notes on Toxoplasma gondii" ' Roy. Soc. Proc.,' B, vol. 87, p. 67. 



EXPLANATION OF PLATES 9 AND 10. 



Figs. 1-3, from Fossa. 



The preparations were photographed under a magnification of 1000 diameters. 

 They were fixed wet in the vapour of iodine dissolved in chloroform, and were stained 

 with Giemsa's stain, made alkaline, and followed by acetone and xylol. 



Fig. 1. — Mononuclear leucocyte in blood from lung, showing enlargement of leucocyte, 

 and the delicate, frothy protoplasm, with hyperchromatosis of the nucleus. The 

 leucocyte contains two parasites, one of ordinary shape just to left of nucleus, and a 

 larger one with undefined extremities at the upper part of the cell. 



Fig. 2. — Two mononuclear leucocytes from the peritoneal exudation, both showing 

 hyperchromatosis of the nuclei. The cells are filled with parasites, and in the cell 

 on the left the nuclei of the parasites are broken up into granules. In this cell a 

 parasite is seen in the nucleus. 



Fig. 3. — A large mononuclear leucocyte from the bone-marrow, with considerable 

 hyperchromatosis of the nucleus, containing a mass of parasites, apparently in 

 process of schizogony, and of breaking up into merozoits. These were found in 

 many stages. 



Figs. 4-6 and 10, from Fruit Pigeon. 



Fig. 4 fixed by the method of Carnoy-Lebrun, and stained with Weigert's hrema- 

 toxylin. Figs. 5 and 6 fixed and stained as Figs. 1-3. Photographed x 1000. 



