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Mr. E. C. Hort. 



invisible, filterable stage, and that this also applies to laboratory cultures 

 of organisms of certain diseases. In passing, I may note that my observations 

 in 1914 in typhus fever, and, later, in the same disease,* in cerebrospinal 

 fever, in scarlet fever and in measles, as to the existence in the infected 

 body fluids of filterable infective viruses, and of growth from these of non- 

 filterable bacteria, has since been confirmed in the case of laboratory 

 cultures of the azotobacteria by Lohnis (2), in the summer of 1916. This 

 observer, however, has recorded no details of his filtration experiments, and 

 does not state if his cultures were from single colonies, or from single 

 organisms, or if he carried out direct observations of growth from single 

 individuals on the warm stage. 



In the present communication I propose to present further pictorial 

 evidence of the complicated life-history of the enteric group of bacteria in so 

 far as this can be studied in laboratory media as opposed to the more natural 

 milieu of infected tissues and body fluids. And to morphological studies of 

 the members of the enteric group I have also added observations on a single 

 strain of a coliform bacillus. 



The strains of organism of the enteric group examined are as follows, their 

 source being also indicated. 



B. typhosus 4 strains : Strain 1, Lister Institute ; Strains 2, 3, 4, Carrier 



strains, Addington. 



B. paratyphosus A 3 strains : Strain 1, Lister Institute ; Strains 2, 3, Carrier 

 strains, Addington. 



B. paratyphosus B 5 strains: Strain 1, Lister Institute ; Strains 2, 3, 4, 5, 



Carrier strains, Addington. 

 B. Shiga-Krme 1 strain : Lister Institute. 



B. Y of Hiss 5 strains: Strain 1, Lister Institute; Strains 2, 3, 4, 5, 



Carrier strains, Addington. 

 B. Fleasmr 3 strains : Strain 1, Lister Institute ; Strains 2 and 3, 



Carrier strains, Addington. 



In figs. 1-4 and Plates 16-19 will be seen the results obtained by study 

 of dried film preparations from young cultures from single colonies, whilst 

 in Plate 20, A, B, C, will be seen growth from single individuals studied on 

 the warm stage. 



All the strains of organisms shown were obtained from the Lister Institute, 

 except the coliform organism. 



It is not possible to reproduce here evidence that the morphological results 

 noted in the case of all the strains enumerated above are identical with the 



* Microphotographic evidence of growth from filterable virus to non-filterable bacteria 

 m typhus fever, together with experimental evidence of pathogenicity at each stage, as 

 well as evidence of complexity of life-history of the enteric organisms, was presented (3) 

 to the Royal Microscopical Society in November, 1916. 



