No. 534] INFLUENCE OF CHANGED CONDITIONS 369 



Diffuse Sunlight. — A considerable number of lots of 

 egg cases and freed cocoons, collected November 4, were 

 placed in diffuse north light in a warm room (21° C.). 

 and all hatched in January and February. 



B. Experiments with Colored Light 

 Freed eggs, collected November 4, were placed within 

 vials immersed in colored solutions within larger bottles, 

 the vial passing through the cork of the larger bottle 

 and held by it. The solutions employed were: acid 

 fuchsine in 50 per cent, and 70 per cent, alcohol; Berlin 

 blue in distilled water; safranine in 95 per cent, 

 alcohol; orange (I in 50 per cent, alcohol; eosine in 70 

 per cent, alcohol; methylen green in distilled water; 

 picric acid in 50 per cent, alcohol; scarlet 12 gin. in 

 1,000 c.c. water, this last giving monochromatic light. 2 

 In Sunlight behind an Alton Filter, then removed to 

 diffuse light in a warm room. (21° C). Lots 43, 46, 47 

 were immersed in a scarlet solution, as follows: 



Lot 43 in sunlight 4 .lays, hatched January 15. 

 Lot 46 in sunlight 28 days, hatched January 9. 



In Diffuse North Light.— The following experiments 

 were made in a breeding room of which the temperature 

 was a few degrees above that out-of-doors. Two differ- 

 ent lots were raised in a fuchsine solution, one in saf- 

 ranine, one in orange (f, one in eosine, one in picric acid. 

 All hatched in May. 



Others were placed in a room at 21° C. One series 

 were immersed in a fuchsine solution for 7, 18, 28, 35 

 days, respectively then removed to ordinary davlight; 

 these hatched in the latter half of January and first half 



in the following solutions: Berlin blue, methyl green, 

 scarlet, and these hatched in the first part of February. 



