On the Diastase o/Kqji. 



307 



In Table III some analyses of the solution of koji, made at the 

 ordinary temperature, are given. They are taken from a large number 

 made, and are selected to show the influence of varying proportions of 

 water used, and also that of length of digestion.* 



Table III. — Composition of the Solution made from 100 grms. of Koji 



at 10° to 15° C. 





Total vo- 

 lume of 

 solution of 



100 grms. 

 of koji. 





Total 



Average 



Glucose 



Specific 

 rotatory 



Average 



JNo. 



Time of 

 digestion. 



solid 

 matter 



percent 

 age of 





per cent, of 

 total solid 



specific 

 rotatorv 





per cent. 





solids. 



matter. 



power p. 





power. 





CO. 



hrs. 









o 



o 







1 



500 



12 



17 7 







60 



65 







2 



1,000 



18 



25 '7 





r 





61 





>57°-6 



3 



>> 



jj 



24 - 2 











55 7 





4 







23 -0 











56 -0 







5 





12 



33 3 









49 



65 3 



-> 





6 



?j 





33 3 









51 -0 



65 -4 







7 



j> 





29 -4 





[>27 -OH 



45 -0 



62 -9 







8 





>> 



28 -6 









46 5 



67 -7 





>64-6 



9 





5J 



26 -8 









53 



61 4 







10 







22 5 









53 -0 



64 5 







11 



5> 



>» 



22 -2 









54 



65 -0 







12 





4 



28 -0 











61 4 





13 



2,000 



3 



31 1 





68 -0 



78 -0 







14 



2,500 



>> 



32 2 







r 



58 -0 



68-1 



■ 





15 





5) 



32 5 





>u + 





70 -0 



65 2 





>69 -3 



16 



)> 



» 



30 -7 







65 -0 



73 -8 





17 







30-1 









68-0 



70 -2 







18 



5,000 



24 



30 -0 







47 -0 



64 5 







19 



10,000 



)} 



40 -0 







66 -0 



60 5 







These examples will suffice to show how variable the amount of 

 solid matter dissolved by the water is, even under apparently the same 

 conditions. Thus when 100 grms. were dissolved in 1,000 cub. 

 centims. of water, and kept for twelve hours at a temperature of 

 10° to 15° C, the amount of solid matter dissolved varied from 22 per 



* The analysis of the solutions of koji was carried out in the following manner. 

 The total solid matter was calculated from the specific gravity of the solution taken 

 at 16° C, compared with water at the same temperature, by dividing the excess 

 above 1,000 (water = 1,000) by 3"86. This gives the number of grms. in 100 cub. 

 centims. of liquid, from which the percentage is easily found. The glucose was de- 

 termined by the gravimetric process recommended by O'Sullivan (" Journ. Chem. 

 Soc," 1876, [ii], p. 131). 



The specific rotatory power of the solid matter in solution was determined by ob- 

 serving the number of divisions upon the scale of a Soleil-Duboscq saccharometer 

 which corresponded to uniformity of tint of the two halves of the field. The light 

 was always observed through a column of liquid 200 millims. long, The specific 



