On the Diastase of Koji. 



309 



Table IV. — Composition of the Solution made from 100 grms. of Koji 

 at Higher Temperatures. 



No. 



Time and temperature 

 of digestion. 



Total volume 

 of solution 

 of 100 grms. 

 of Jcoji. 



Total solid 

 matter per 

 cent, of koji 

 used. 



Dextrose 

 per cent, of 

 total solid 

 matter. 



Specific 

 rotatory 

 power p. 



1 



2 

 3 

 4 

 5 



2 hours at 50° C. + 18 



cub. centims. 



| 1,700 



2,000 

 2,000 

 5,000 



| 10,000 



51 -8 



31-8 

 61 6 

 37 2 



49 -2 



68 -0 



84 -9 

 .68 -5 

 66 -0 



58-0 



o 



68 



76 1 



53 5 

 63 2 



73-8 



f hour at 45° -C 





i hour at '50° C 



24 hours at 15° + 2 

 hours at 100° C 





The percentage of solid matter dissolved out is greater in most of 

 these experiments than in those conducted at the ordinary temperature. 

 Experiments 2 and 3 (Table IV) are interesting as they were made at 

 the same time, with the same specimen of hcji, and with the same pro- 

 portion of water, the only difference being in the time of digestion. It 

 will be useful to compare them with the results of an experiment 

 made with the same Jcoji at the ordinary temperature of the air. 100 

 grms. of the Jcoji were digested with the 2,000 cub. centims. of water 

 at 10° to 12° C. for eighteen hours. The results of the three experi- 

 ments are grouped together in Table V. 



Table V. 



18 hours at 10—12° C. . . . 



Total solid 

 matter per 

 cent, of 

 koji. 



Dextrose 

 per cent, 

 of koji. 



Dextrose 

 per cent, of 

 total solid 

 matter. 



Specific 

 rotatory 

 power p. 



29 -2 

 31 8 

 61'6 



20-25 

 27-00 

 42 -20 



69 3 

 84-9 

 68-5 



66°3 

 76-1 

 53 -5 







There is not much difference in the amount of solid matter dis- 

 solved out in the two first experiments, but the proportion of dextrose 

 contained in the second is very much higher than in the first. In the 

 third, the total solid matter has increased very greatly, but the dextrose 

 dissolved has not kept pace with it, and forms only the same propor- 

 tion as in the solution made in the cold. The lower specific rotatory 

 power shows, however, that the proportion of the other constituents 



