420 Dr. F. W. Pavy. Research bearing on the [June 16, 



In some instances, the process of preparation for titration with the 

 test consisted of extraction with alcohol, and in others of precipitation 

 of the albuminous and colouring matters by heat with the aid of 

 sulphate of soda. Bernardin (glycogen) prepared in different ways 

 was used, and no difference in the results was perceived. Some 

 of the specimens employed were obtained by simple extraction 

 from the liver with water and precipitation with spirit. The actual 

 condition of the specimen used as regards purity was ascertained 

 by subjection to the converting action of sulphuric acid and heat, 

 and the estimation of the glucose formed. In this way, the in- 

 formation was obtained for supplying the figures found in the sub- 

 joined table representing the Bernardin used, expressed in its equiva- 

 lent of glucose. The figures representing the sugar produced were 

 obtained by deducting from the sugar found in the product of experi- 

 ment the figures yielded by a specimen of the blood alone purposely 

 exposed to parallel conditions. 



Bernardin (glycogen) added to Blood and exposed for half an hour 

 to a temperature of 100° to 110° F. 



Experiment 1. 



2. 



3. 



4. 



5. 



6. 



7. 



8. 



9. 

 10. 

 11. 

 12. 

 13. 

 14. 

 15. 

 16. 

 17. 



Amount of blood 

 used. 



cub. centims. 

 60 

 60 

 30 

 30 

 25 

 50 

 50 

 50 

 50 

 50 

 50 

 50 

 25 

 50 

 25 

 25 

 25 



Amount of sugar 

 producible from 

 the Bernardin 

 mixed with the 

 blood. 



grm. 

 0-267 

 0-267 

 0-535 

 0-535 

 0-232 

 0-432 

 0-288 

 0-144 

 0-294 

 0-147 

 0-144 

 0-144 

 0-144 

 0-144 

 0-144 

 112 

 0-112 



Sugar produced. 



yrm. 

 •007 

 •018 

 •005 

 •005 

 •009 

 •014 

 •021 

 •007 

 •009 

 •009 

 •036 

 •033 

 •007 

 •009 

 •000 

 •003 

 •002 



p. 272. Since bringing it under the notice of the Society, I have had a very 

 large experience with it, and am thus enabled to speak in definite and confident 

 terms about it. Its facility of application, delicacy, and precision place us in a most 

 advantageous position in relation to the quantitative determination of sugar. An 

 important feature also belonging to it is that its action is not interfered with by the 

 presence of nitrogenous matter as is the case with the ordinary cupric solution. 

 Under my supervision it has been used, I am quite within bounds in saying, some 



