1881.] Physiology of Sugar in the Animal System. 



429 



subjected to the several daily washings was boiled with potash and 

 the liquefied product poured into spirit and the Bernardin collected 

 :and estimated. The following are the figures that were yielded : — 



Per 1,000 of liver. 



Bernardin extracted the 1st day 13'833 



2nd „ 3-879 



3rd „ 2-961 



4th „ 2-817 



Bernardin remaining in the liver residue . . 35 "145 



In another similarly conducted experiment upon the liver of a cat 

 the following results were obtained : — 



Per 1,000 of fiver. 



Bernardin extracted the 1st day 3-951 



2nd „ 1-134 



„ ? , 3rd Trace 



Bernardin remaining in the liver residue . . 3 - 609 



The above liver, it will be seen, was poor in Bernardin ; and this is 

 -often noticed in the cat, unless the animal has been fed with milk or 

 bread in addition to, or in lieu of, meat. 



In a third experiment upon the liver of a rabbit the following 

 figures were furnished : — 



Per 1,000 of liver. 



Bernardin extracted the 1st day 13'503 



2nd „ 3-376 



4th „ 3-688 



8th „ 2-466 



12th „ 2-299 



Bernardin remaining in the liver residue . . 73*167 



These results show how imperfectly ordinary extraction with water 

 removes Bernardin from the liver substance, and account for the small 

 quantities which, perhaps, may have been noticed to have been 

 obtained when aqueous extraction has been had recourse to for collect- 

 ing it. 



Before the above quantitative experiments were performed, I noticed 

 the following circumstance, which, indeed, led me to undertake them. 

 Some bruised liver substance was washed in a beaker with boiling 

 water, till the water failed to present the slightest appearance of 

 lactescence. The last washing was poured off, and the liver substance 

 simply left in a moist condition in the beaker, till the following day. 

 On then boiling it up with water, a highly lactescent liquid (from the 

 ■extraction of Bernardin) was again obtained. A few repetitions of the 

 washing resulted, as on the previous day, in clear liquid being yielded, 

 and the residue placed aside as before in a moist state, gave rise on 



2 g2 



