On a New Form of Febrile Disease. 



497 



Pathological Results. 

 First Series of Experiments. 



a. Inoculated rat with distilled water. No result. 



b. Inoculated rat with milk from Cowie Dairy. No result. 



c. Inoculated rat with cultivated milk from Cowie Dairy. No 

 result. 



d. Inoculated rat with milk from Oldmill Dairy, taken on 27th 

 April. No result. 



e. Inoculated rat with milk, 27th April, after cultivation. No result. 

 /. Inoculated rat with Oldmill milk of 3rd April, containing 



spores, micrococci, and Torulse. 



g. Inoculated rat with milk of 3rd April, after cultivation, contain- 

 ing bacilli, short filaments, micrococci, and Torula?. 



Death followed in both cases from fifteen to twenty hours after in- 

 oculation. After several failures, I succeeded in obtaining a cultiva- 

 tion from near the seat of inoculation, containing bacilli only, or 

 at least very few micrococci. In due time the bacilli developed into 

 spore- bearing filaments. 



Inoculation with the spores from this cultivation produced the same 

 result as the milk and the milk cultivations / and g, but the spores 

 were slower in their action, and now (7th June) the fatal result does 

 not follow until four days after inoculation with cultivated spores. 



Second Series of Experiments. 



Other rats were inoculated with the fresh hay infusion, with the 

 turnip infusion, with an infusion of grains, with the Oldmill hay 

 infusion, and with distilled water. No result followed except in one 

 instance. A rat, twenty-five hours after inoculation with the Old- 

 mill hay infusion, was found dead. I have not had time to complete 

 this series of experiments. 



Third Series of Experiments. 



From one of the cases in which the induration in the neck ended in 

 suppuration, I obtained a small quantity of pus, antiseptically 

 collected and sealed in vaccine tubes. On examination the pus was 

 seen to contain a number of bacilli and spores, which exactly 

 resembled the spores previously found in the 3rd April Oldmill milk. 

 On placing a very small drop of this pus in a cell and introducing 

 the cell into a warm chamber, the spores germinated and some of the 

 rods lengthened into filaments, but the filaments have not yet (I3th 

 June) developed spores. 



Pus collected in the same way, but apparently containing neither 

 bacilli nor their spores, has not, under cultivation, undergone any 

 change. 



