Colouring-matter of Human Urine. 



27 



has become yellow, nothing font the band at F is left. Jaffe ("Zeitsch. 

 f. Chem.," v, 666) succeeded in isolating the pigment which gives the 

 feeble bands on each side of D, and also that which gives the band at F. 

 The pigment which gave this last band, when isolated, was brown-red 

 in colour, soluble in alcohol, ether, and chloroform; the solutions 



Figure reduced from Chart I. (See p. 36.) 



being a fine red colour, giving, when acidulated, a dark band at F. 

 By treating a solution of dog's bile with hydrochloric acid, the same 

 observer obtained a red fluid, which became yellow on the addition 

 of alkalies, and which, before alkalies were added, was marked by 

 giving the same dark band as before, but after the addition of alkalies, 

 especially caustic soda, this band was replaced by another one nearer 

 the red. Since Jaffe described these appearances, Maly* has asserted 



* " Ann. Ch. Pharm.," chri, p. 368 ; elxiii, p. 77. t 



