Colouring-matter of Human Urine. 



31 



Ethereal Solution of Urobilin, got by Sulphuric Acid and Alcohol 

 Method described above. — The colour is dark red. 



Spectrum — 



Extent, 10 to 65 (or wave-length 678— 457). \ 

 Band 21 to 23 (or wave-length 604-592.) I g ee fi 9? (2) 



Band S, 28 to 32 (or wave-length 568— 552). f ° / 

 Band a, 45 to 55*5 (or wave-length 507—479) 'J 



By treating an appropriate depth of this fluid in a cell with am- 

 monia, the colour becomes lighter and more yellow, and gives the 

 spectrum shown in figure (3). At a less depth, the band at E is seen to 

 have disappeared. This band shown in the figure (2) reads from 

 23 to 29 (or wave-length 592—564). 



When excess of ammonia was removed by means of a small pipette, 

 and the solution was acidified with acetic acid, the band at F re- 

 appeared. 



Alcoholic Solution of same Pigment. — This solution was of a red 

 colour, and gave, when examined at a suitable depth, two bands, and 

 at a less depth another black band at F. 



The readings of the bands at the first depth were as follows : — 



Extent, 9 to 37 (or wave-length 686—534) "] 



Band e , 21 to 23 (or wave-length 604—592) >See figure (4). 



Band c, 26 to 29 (or wave-length 578—564) J 



Less depth : — 



Extent, 9 to 78 (or wave-length 686— 435) 1 g fi 



Band a, 45 to 55 (or wave-length 507—480) J 66 §Ure { ° } ' 



When the first depth was treated with ammonia, a band appeared 

 reading 25 — 29 (or wave-length 582 — 564), and in a slight depth of* 

 fluid so treated the band at E had disappeared. 



This solution then gives the same spectrum as the ethereal solution, 

 the slight differences in positions of bands being due to different re- 

 fractive power of solvent used, and when ammonia was added, the 

 same effect as in the case of the ethereal solution was produced. See 

 figure (6.) 



Chloroformic Solution. — Bed solution, giving in sufficient depth two 

 bands, and in less depth, one band. 



The readings of the spectrum of first depth were as follows : — 



Extent, 11 to 40 (or wave-length 671—523) 1 



Band e, 21 to 23'5 (or wave-length 604—590) > See figure (7). 



Bando, 27 to 31 (or wave-length 573— 556) J 



The band of the shallower depth read from 44 to 55 (or wave- 

 length 510—480). See figure (8). 



Treated with ammonia, I got the band shown in figure (9), reading 



