34 Dr. C. A. MacMunn. Researches into the 



Ethereal solution. — Of an orange colour. It gave the following 

 spectrum when treated with ammonia : — 



Extent, 10 to 37 (or wave-length 678—534)1 



Band B, 16 to 20 (or wave-length 634—609) > See figure (16). 



Band 7 , 23 to 28 (or wave-length 592—568) J 



In a less depth the band « at F of original solution had disappeared. 



Chloroformic solution. — This solution gave a similar spectrum to, and 

 was altered similarly by, ammonia as the other solutions just described. 



Action of Caustic Soda on these solutions. — In appropriate depths 

 caustic soda moves the band at F nearer the red. Thus in the case of 

 the alcoholic solution : — 



Band before treatment with caustic soda, 47 to 53 (or wave-length 

 502—485). 



Band after treatment with caustic soda, 40 to 45*5 (or wave-length 

 523—506). 



And the action of caustic soda on the ethereal and chloroformic solu- 

 tions was similar. When urobilin is thus treated with caustic soda, 

 ammonia is no longer capable of causing the band to disappear. 



Action of Acids on Urobilin prepared as above. — It dissolves in sul- 

 phuric acid, giving a splendid ruby-red solution, having the following 

 spectrum : — 



Extent, 10 to 40 (or wave-length 678—523). 

 As the bands are uncertain, alcohol was added, when a band appeared 

 from 



21 to 25 (or wave-length 604—582), 

 and a very faint shading from 



30 to 35 (or wave-length 560—542) see figure (12). (Band at F in 

 shallow depth.) 



It dissolves in acetic acid, forming an orange-red solution, giving in 

 suitable depths the following spectrum : — 



Extent, 10 to 38 (or wave-length 678—530)] 



Band£, 22 to 26 (or wave-length 598—578) >See figure (17). 



Band 7, 32 to 35 (or wave-length 552—542) J 



Shallow depths : — 



Extent, 8 to 85 (or wave-length 694—425). 

 Band a,, 45 to 55 (or wave-length 507 — 480). 



Ammonia develops in this solution three feeble bands : — 



Extent, 10 to 41 (or wave-length 678—520). 

 Band e, 8 to 11 (or wave-length 694 — 671). 

 Band w, very feeble, reading could not be 

 taken. 



Band h, 34 to 37 (or wave-length 545—534). 



