1880.] 



Colouring-matters of Human Urine. 



235 



Spectrum 12. Pigment got by action of sodium amalgam on bilirubin dissolved in 

 alcohol, and precipitated by means of hydrochloric acid.* 

 13. The same, thin layer (the band at F is shown rather too dark.) 

 „ 14. The same treated with caustic soda.f 



„ 15. Alcoholic solution of chloroformic residue of human bile pigments, 

 deep layer ; in a thinner layer another band is seen wave-length 504 

 to 480. 



„ 16. Ditto, treated with zinc chloride. 

 ,, 17. Ditto, treated with hydrochloric acid. 

 „ 18. Thin layer of the same. f 

 Compare 2, 7, 12, and 17, and 3, 14, and 16. 



Chaet III. 



Spectrum 1. 



Solar spectrum. 



33 



2. 



Ox-bile. 



JJ 



3. 



Chloroformic solution pigments of ox-bile. (Compare 2 and 3 with 20 r 







infra.) 





4. 



Pigments of ox-bile in alcohol. 





5. 



The same treated with caustic soda. 



33 



6. 



Action of chloride of zinc on alcoholic solution (4) . 



>J 



7. 



The same, shallow depth. 



33 



8. 



The same (7) treated with hydrochloric acid. 





9. 



Hsematoin (acid hseruatin) in alcohol. 



» 



10. 



Hsematoin treated with zinc and sulphuric acid and filtered. (Compare 







Chart 1, 11, 12, 14.) 



» 



11. 



The isolated urohaematin in alcohol. 



33 



12. 



The alcoholic solution with caustic soda. 



33 



13. 



The alcoholic solution with zinc chloride. 



» 



14. 



The artificially prepared urohsematin in chloroform. 





15. 



Action of peroxide of hydrogen on hsematoin. 



33 



16. 



The pigment produced by oxidation isolated and reduced by sodium 







amalgam and filtered. 



33 



17. 



This filtrate treated with sulphuric acid. 



33 



18. 



Spectrum produced by the action of decolorised bile on haemoglobin. 





19. 



The same or a similar spectrum, got by acting on blood with alcohol and 







caustic soda. 



» 



20. 



Spectrum closely resembling sheep or ox bile, produced by the action of 



sodium amalgam for half-an-hour in the cold, on the body whose 

 spectrum is shown in 19. (Compare 2 and 3, supra.) 



Chaet IV. 



Spectrum 1. Solar spectrum. 

 „ 2. Sheep-bile. 



„ 3. Pigments of sheep-bile in chloroform. 

 ,, 4. Pigments of sheep-bile in alcohol. 



„ 5. Bands of heemochroniogen, got by adding sulphide of ammonium to 

 fluid giving 4. 



* And dissolved in alcohol. 



f This also represents solution of which sp. 15 is the map treated with caustic 

 soda. 



