326 



Prof. H. C. Bastian on the Heat 



the heat to which the mixture has been exposed may have been adequate 

 to kill all the living units entering into the inoculating compound, 

 although it may not have been sufficient to prevent its not-living organic 

 matter acting as a ferment upon the infusion*. 



And there are, I think, the very best reasous for concluding that in all 

 the cases in which turbidity has occurred after the organic mixtures have 

 been subjected to a heat of 140° E. (60° C.) and upwards, this turbidity 

 has been due, not to the survival of the living units, but rather to the 

 fact that the mere dead organic matter of the inoculating compound has 

 acted upon the more unstable organic infusions in a way which it was 

 not able to do upon the boiled saline fluids. 



In order more fully to explain the grounds upon which this conclusion 

 is based, it will now be necessary to recast the results of the 102 inocu- 

 lation experiments recorded in my last communication f . They require 

 to be thrown into a new tabular form, in order to show how the results 

 differed amongst themselves when organic infusions of different strengths 

 were employed. The consideration of this aspect of the question was 

 purposely .delayed, in order to avoid the introduction of unnecessary 

 complications into my last communication, seeing that the conclusion 

 which I then sought to establish was in no way affected by these facts. 



Neutral Hay-Infusion. 



Infusion of sp. gr. 1005. 



Infusion of sp, gr. 1002. 



Tempe- 

 rature. 



Number 



of 

 Expts. 



Date of 

 Turbidity, 

 if any. 



Results at the 

 Expiration of 

 the 8th day. 



; Number 

 I Expts. 



Date of 

 Turbidity, 

 if any. 



Results at the 

 Expiration of 

 the 8th day. 



122° F. 

 (50° C.) 

 131 °F. 



} 1 



6 



24 hrs. 

 48 hrs! 



Turbid. 

 All turbid. 



1 



48 hrs. 



Turbid. 



140° F. 

 (60° C.) 



149° F. 



} 7 { 



• 



1 in 48 hrs. 

 6 in 60 hrs. 



| All turbid. 



1 •} 



1 in 3 days. 



1 in 8 days. 



2 in 5 days. 

 1 in 8 days. 



| All turbid. 



Three turbid. 

 One clear. 



158° F. 

 (70° C.)- 

 167° F. 

 176° F. 

 (80° C.) 



}...... 



), 





All clear. 



15 

 4 





All clear. 

 All clear. 



* See ' The Beginnings of Life,' vol. ii. p. 2. 



f See Proceedings of the Royal Society, No. 143, p. 230. 



