necessary to kill Bacteria, Vibriones, fyc. 327 



Acid Turnip-Infusion. 



Infusion of sp. gr. 1008. 



Infusion of sp. gr. 1005. 



Tempe- 

 rature. 



Number 



of 

 Expts. 



Date of 

 Turbidity, 

 if any. 



Results at the 

 Inspiration of 

 the 8th day. 



Number 



of 

 Expts. 



Date of 

 if any. 



Results at the 

 Expiration ol 

 the 8th day. 



122° F. 

 131° F. 



5 



24 hrs. 



All turbid. 



2 



48 hrs. 



All turbid. 



140° F. 

 149° F. 



3 



40 hrs. 

 5 days. 



All turbid. 

 All turbid. ! 



« { 

 M 



4 in 3 days. 

 2 in 4 days. 

 1 in 3 days. 



1 in 7 days. 



2 in 8 days. 



| All turbid. 



I Four turbid, 

 j Three clear. 



158° F. 

 167° F. 

 176° F. 







: . 1 



17 

 4 





All clear. 

 All clear. 



Reference will be made to these Tables in the setting forth of my 

 reasons for the conclusion that the more or less delayed putrefaction which 

 takes place in inoculated organic infusions raised to the temperature of 

 140° F., and to other degrees of heat above this point, is due to the in- 

 fluence of the not-living ingredient (b) of the inoculating compound. 

 These reasons are the following : — 



1. Because the turbidity which has occurred in inoculated organic in- 

 fusions that have been subjected to a temperature of 140° F. has always 

 manifested itself appreciably later, and advanced much more slowly than 

 in similar mixtures which had not been heated above 131° F. ; whilst it 

 has commenced even later, and progressed still more slowly, when occur- 

 ring in mixtures previously heated to 149° F. Such facts might be 

 accounted for by the supposition that exposure in these organic fluids 

 to the slightly higher temperature suffices to retard the rate of growth 

 and multiplication of the living units of the inoculating compound, al- 

 though the facts are equally explicable upon the supposition that the 

 later and less energetic putrefactions are due to the sole influence of the 

 mere organic matter of the inoculating compound. 



2. So far as the evidence embodied in the Tables goes, it tends to show 

 that the more unstable different specimens of similar infusions are (that 

 is, the stronger they are), the more rapidly and frequently does late tur- 

 bidity ensue, and the more this late turbidity approaches, both in time 

 of onset and in rate of increase, to that which occurs when inoculated 

 infusions are not heated to more than 131° F. — when both living and 

 not-living elements of the inoculating compound act conjointly as 

 ferments. Such facts show quite clearly that where the intrinsic or 

 predisposing causes of change are strong, there less potent exciting 

 agencies are more readily capable of coming into play ; but they still do 



2e2 



