1873.] 



of Bacteria in Organic Li fusions. 



353 



yielded no indication whatever of a development of Bacteria or other 

 organisms, nor of any change. A portion of the same infusion placed in 

 an uucleansed beaker for comparison was milky and swarming with 

 Bacteria after three days. 



Seeies C. Nov. 28th. Experiments with tumip-and-cheese infusion. — 

 The infusion similar in all respects to that in series B, but prepared with 

 a somewhat larger proportion of turnips ; therefore of higher specific 

 gravity, which was not numerically determined. 



Tubes 20, 21, 22, 23. Boiled and sealed approximately during ebullition. 

 Not subsequently submerged. 



Tubes 24, 25. Boiled and sealed approximately during ebullition. 

 Subsequently submerged in boiling water during thirty minutes. 



The tubes were preserved in the air-bath as in Series A and B. 



Seeies D. Nov. 30th. — An infusion prepared as in Series B and C, but 

 brought to a sp. gr. 1031 by evaporation after filtration. 



Tubes 26, 27, 28, 29. Sealed cold. Subsequently submerged in boiling 

 water for thirty minutes. 



Tubes 30, 31. Boiled and sealed approximately during ebullition. Not 

 subsequently immersed. 



Tubes 32, 33. Boiled and sealed approximately during ebullition. Sub- 

 sequently submerged in boiling water for thirty minutes. 



Appearances in the infusions, Series C and B, at the time of sealing and 

 submerging. — The appearances in the freshly prepared infusion were 

 similar to those described above as characterizing such infusions. 



Subsequent naked-eye examination of the tubes did not reveal the 

 slightest change ; they remained limpid. Specimens from each group 

 were opened and examined with the microscope after four days, and the 

 microscopic characters found to be unchanged : the liquid was perfectly 

 sweet. The remaining tubes were examined at intervals before the end 

 of December, being maintained during the whole time at a temperature 

 of 35° to 40° C. in the air-bath ; they equally proved to have remained 

 unchanged when opened and examined with the microscope, and were 

 also free from unpleasant smell. 



Seeies E. Nov. 28th. — Six porcelain capsules were heated to redness, 

 and nearly filled with the turnip-infusion used in Series C. They were 

 placed on the air-bath under a glass shade. 



Capsules 1, 2. The infusion was unboiled. 



Capsule 3. The infusion was boiled in the capsule. 



Capsule 4. The infusion was introduced after it had been boiled for five 

 minutes in a superheated test-tube. 



Capsules 5 and 6. The infusion was that used in capsule 4, but a drop 

 of distilled water was added to each of these two capsules. 



After four days the infusion in capsules 1, 2, 5, and G was found to be 

 teeming with Bacterium termo and Bacterian filaments. 



Capsule 3 was found to be cracked, and hence was discarded (it 

 swarmed with Bacteria). 



