356 Messrs. Pode and Lankester on the Development [June 19, 



which small lumps of cheese were present during the subjection of the 

 flask to the process of ebullition and subsequent immersion in boiling 

 water. 



This result induced us to make a further series of differential experi- 

 ments, bearing upon the influence of the state of aggregation of the 

 cheese introduced into the turnip-infusion. 



Series H. March 8th. — A turnip-infusion was prepared as in Series B ; 

 found after filtration to have sp. gr. 1113-5. 



Tubes similar to those used in Series A-E, and half filled, were used. 



Tubes 42, 43, 44. The simple infusion was poured into the tube, so 

 as to half fill it ; a lump of cheese the size of a pea was then added. 

 Sealed cold. 



Tubes 45, 46, 47. To the turnip-infusion, before introduction into the 

 tubes, an emulsion of cheese prepared with turnip-infusion and strained 

 through a piece of cambric was added. The tubes were then half filled 

 with this mixture and sealed cold. 



Tubes 48, 49, 50. The same as 42, 43, 44, but sealed approximately 

 during ebullition. 



Tubes 51, 52, 53. The same as 45, 46, 47, but sealed approximately 

 during ebullition. 



All the tubes, 42 to 53, were completely submerged during five minutes 

 in boiling water, and subsequently preserved in the air-bath at 35° C. 

 temperature. 



On March 13th the contents of the twelve tubes were examined with 

 the microscope. No. 45 had been broken in the boiling. The five 

 remaining tubes which had been prepared with cheese in the finely 

 divided condition were found to be entirely devoid of life, the infusion 

 microscopically and-' otherwise unchanged. Of the six tubes prepared, 

 each with a small lump of cheese, no organisms were detected in 42 and 

 44 ; but in 43 and 49 a few elongate Bacteria were observed (in the 

 proportion of about two to the field of a Hartnack's system 10). In 48 

 and 50 the fluid was swarming with elongate Bacteria and true Bacillus. 

 The lumps of cheese in those tubes in which life appeared had softened 

 and spread out to a certain extent on the side of the tube. The cheese- 

 lumps in Nos. 42 and 44 retained their original form. 



From the result of these later experiments, made in consequence of 

 the fuller information given by Dr. Sanderson as to Dr. Bastian's mode 

 of treating turnip and cheese so as to obtain phenomena supposed to be 

 in favour of the doctrine of Archebiosis, we consider that the importance 

 of excluding visible lumps from the experimental infusions is clearly 

 indicated, as also is the comparatively greater trustworthiness of the small 

 tube as opposed to the larger retort for use as an experimental vessel. 

 We moreover consider that we, in our earlier experiments (November 

 and December), carefully following Dr. Bastian's directions, as far as he 



