188 Dr. F. Crace-Calvert on Prevention of Putrefaction. [Feb. 29, 



time to time for eighty days. During this time the tubes were kept in a 

 room the temperature of which did not vary more than 3 degrees, namely, 

 from 12-5° C. to 15\5° C. 



In order the better to show the influence of the antiseptics used, I ex- 

 amined two specimens of the same solution at the same time, one of which 

 was kept in the laboratory, the other in the open air. 

 . A marked difference was observed in the result, the one kept outside 

 becoming impregnated with animal life in less than half the time re- 

 quired by the other, while as many vibrios were developed in six days in 

 the tube kept outside as were developed in thirty days in the tube in the 

 laboratory. 



A summary of the results of the experiments is given in the following 

 Table, in which the substances are grouped according to their chemical 

 nature : — 



Days required for deve- 

 lopment of 



1. Standard Solutions. 

 Albumen kept in laboratory for comparison 



Sulphurous acid 

 Sulphuric acid. . 

 Nitric acid 

 Arsenious acid. , 

 Acetic acid 

 Prussic acid. . . . 



I\mgi. 

 18 



Albumen exposed outside laboratory None 



2. Acids. 



21 



9 



10 



18 



9 



None 



3. Alkalies. 



Caustic soda 18 



Caustic potash 16 



Caustic ammonia 20 



Caustic lime None 



4. Chlorine Compounds. 



Solution of chlorine 



Chloride of sodium 



Chloride of calcium 



Chloride of aluminium 



Chloride of zinc 



Bichloride of mercury 



Chloride of lime 



Chlorate of potash 



5. Sulphur Compounds. 



Sulphate of lime 



Protosulphate of iron 



Bisulphite of lime 



Hyposulphite of soda 



Vibrios. 



12 



5 



11 



9 



10 



22 

 30 

 9 



24 

 26 

 24 

 13 



22 



7 



19 



14 



18 



7 



21 



10 



53 



None 



81 



None 



16 



9 



19 



17 



19 



9 



15 



7 



18 



11 



18 



11 



