1886.] 



On the Multiplication of Micro-organisms. 



535 



colony when young has a very characteristic appearance, resembling a 

 spherical or ovoid sea-urchin covered with spines, and light brown or 

 gray in colour. 



o 



Magnifying power nearly 1000. Magnifying power about 100. 



2. Appearance of the Finkler- Prior s Comma Spirillum in Gelatine- 

 tube Cultivations. 



The growth of this organism very much resembles that of the 

 Bacillus pyocyaneus, only that no colouring-matter is produced. The 

 liquefaction, which is at first funnel-shaped, rapidly extends across 

 the whole tube, and the downward extension into the solid gelatine 

 becomes filled with a plug of a yellowish viscid material. In the 

 course of a few weeks the whole gelatine, or at least as far as the 

 needle at the time of inoculation has reached, becomes liquid, the 

 yellowish precipitate resting on the bottom, but the liquefied portion 

 also remains turbid. 



Appearance of the Finkler-Prior's Comma Spirillum in Gelatine-plate 



Cultivations. 



The plate cultivations of this organism are very characteristic. The 

 colonies making their appearance already on the first or second day 

 as small milky white disks, which increasing in size cause rapid 

 liquefaction of the gelatine, the depressions being filled with a white 

 fluid resembling thin milk. If the centres are at all closely approxi- 

 mated they very soon become confluent, and lose their identity, the 

 whole plate becoming fluid. 



Viewed under the low power of the microscope the colonies 

 appear as brownish very finely granular disks, with a highly refract- 

 ing edge, which is also granular and not perfectly sharp. 



K- /.l Hi 



Magnifying power nearly 1000. Magnifying power about 100. 



3. Appearance of Koch's Comma Spirillum in Gelatine-tube 

 Cultivations. 



The growth of this organism in gelatine is slower than that of the 

 other two. At 20° C, within a day or two of inoculation, the path of 



