540 



Dr. P. F. Frankland. 



[June 10, 



Comma Spirillum {from weak cultivation in Gelatine) in Sewage and, 



Deep Well-water. 



Number of Colonies obtained from 1 c.c. 



No. n r 



„ 2T Sewa s e j 



I 41 r 

 1 Deep well J 

 „ 5 [ water ] 

 „ 6j L 



Day of 

 preparation. 

 — 



2nd Day. 



4th Day. 



525 



525 

 525 

 395 



395 

 395 







(Incub.) 

 

 

 



(Incub.) 

 

 







(Incub.) 

 

 

 



(Incub.) 

 

 



These experiments show that in every case the comma Spirilla from 

 this weak cultivation lost their vitality in twenty-four honrs when 

 introduced into these dilute media. The one case (No. 6 in distilled 

 water) in which they were subsequently demonstrable, differed from 

 the others, inasmuch as this was the bottle in which the cultivation 

 was first attenuated, in fact Nos. 1, 2, and 3 were prepared by dilu- 

 ting No. 6 about 300 times. But even in this first attenuation the 

 vitality of the organism is seen to be destroyed within nineteen 

 days, and similarly the first attenuation, from which the six last 

 experimental bottles above were inoculated, was also examined after 

 nine days, and the comma Spirilla no longer found demonstrable. 



In the following experiments the comma Spirilla employed were 

 taken from a vigorous cultivation in peptone-broth, the results ob- 

 tained being as follows : — 



