1878.] 



Etiology of Infectious Diseases. 



105 



The preparation was then kept in the incubator for twenty-four hours 

 at a temperature of 32-33° C. After this time it was used to in- 

 oculate a new drop of humor aqueous in a similar manner as the one 

 just described. We will call this the second generation. 



This new specimen was placed in the incubator and kept there at a 

 temperature of 32-33° C, for further twenty-four hours. In the 

 same manner a third generation was started by inoculating a fresh 

 drop of humor aqueous. After having been kept in the incubator 

 for several days it was used to inoculate two animals at different times. 

 Both animals became smitten with the disease. 



(&.) The other five experiments were carried out with virus culti- 

 vated from solid lymph of the peritoneum of a diseased animal. The 

 lymph had been dried at 38° C (See Series 2). A small particle of 

 dried lymph is crushed into fine powder. With a granule of this a 

 drop of fresh humor aqueous is inoculated in the same manner as above 

 described. — First generation. 



After having been kept in the incubator for two or three days at a 

 temperature of 32-33° C, it is used to inoculate a second generation, 

 care being taken to use a trace only of the fluid part and not to come in 

 direct contact with the original granule, which may be still discerned 

 in the preparation. 



The specimen representing the second generation is kept in the in- 

 cubator for a day or two. It is then used to inoculate a fresh prepa- 

 ration. — Third generation. And, finally, this is used for establishing 

 a fourth generation. After having been kept in the incubator, a part 

 of it is used for inoculating two animals, the inoculation being carried 

 out at different times. Both these animals become smitten with the 

 disease. 



Another portion of this fourth generation was used to start a fifth 

 generation, then a sixth, a seventh, and an eighth generation. With 

 this three animals were inoculated at different times. All three 

 animals became diseased in due time. 



In order to correctly interpret the results of this last (6th) series of 

 experiments, it is important to mention that inoculation with dried 

 lymph, diluted far less than would correspond to the third generation 

 in the last-named experiments, is followed by a negative result. 



The microscopic examination of the cultivated liquids proves that 

 these are the seat of the growth and development of a kind of bac- 

 terium, which has all the characters of bacillus subtilis (Cohn). The 

 bacillus in our case is a very fine and delicate rod, thinner than both 

 that described by Professor Cohn in hay-infusion, and the bacillus 

 anthracis so thoroughly investigated by Dr. Koch. 



Our bacillus differs also in other respects from that of bacillus an- 

 thracis, inasmuch as it possesses a moving stage, the bacillus anthracis 

 described by Dr. Koch is non-moving. Like bacillus subtilis of hay 



