1879.] Physiology of Sugar in relation to the Blood. 521 



The figures in the next two columns represent the results given by 

 the same two processes of analysis applied to the product obtained after 

 the plan adopted for the gravimetric process. The blood is treated 

 with sulphate of soda, filtered, the coagulum thoroughly washed to 

 extract all the sugar, and the filtrate and washings brought to a known 

 volume. 



The last column furnishes the mean of two gravimetric analyses 

 carried out upon two portions of the blood distinct from that employed 

 for the analyses in columns 3 and 4. 



Results given by Bernard's, the Ammoniated Cupric, and the Gravi- 

 metric Processes for the quantitative determination of Sugar in 

 Blood. 



Source. 



Sugar per 1,000 parts. 



With Bernard's formula 

 for estimating volume 

 of liquid derived from 

 the blood. 



Preparation of blood 

 product by the pro- 

 cess employed for the 

 gravimetric method. 



Grravimetric 



process. 

 Mean of two 

 analyses. 



Bernard's 

 potash 

 process. 



Ammoniated 

 cupric 

 process. 



Bernard's 

 potash 

 process. 



Ammoniated 

 cupric 

 process. 



I. Sheep .... 



0-930 



0-560 



0-842 



0-571 



0-589 



II. Bullock .. 



1-212 



901 



0-980 



650 



735 



III. Bullock .. 



1-568 



1-130 



1-240 



0-896 



921 



IV. Sheep .... 



0-888 



0-579 



0-905 



0-567 



0-533 



V. Bullock . . 



0-816 



0-534 



0-839 



0-559 



0-511 



VI. Sheep .... 



0-879 



0-635 



0-945 



0-650 



0-631 



On looking at the results the first point to which, attention may be 

 directed is that evidence is supplied showing that reliance cannot be 

 placed upon the formula adopted by Bernard for calculating the volume 

 of liquid derivable from the weight of blood taken for analysis. The 

 figures in columns 3 and 4 were drawn from direct observation, and if 

 the formula supplied correct information, the results in columns 1 and 

 3 and 2 and 4 should respectively coincide. It is noticeable, however, 

 that whilst in some instances they approach closely towards agreement, 

 in others there is a pretty wide divergence. 



In the second place it is seen that the results obtained by the method 

 of analysis involving the employment of the potash stand considerably 

 higher than those yielded by the ammoniated form of the test. It 

 may be assumed that in the action of the potash on the incidental 

 organic matter present to give rise to the required condition for main- 



