﻿118 
  

  

  Dr. 
  R. 
  C. 
  Shettle 
  on 
  the 
  

  

  [Dec. 
  10, 
  

  

  The 
  store 
  bottles 
  (fig. 
  5) 
  were 
  narrow-mouthed, 
  capable 
  of 
  holding 
  a 
  

   pint 
  ; 
  they 
  were 
  closed 
  with 
  a 
  cork 
  with 
  one 
  glass 
  tube, 
  fitted 
  with 
  india- 
  

  

  Fig. 
  4. 
  Tig. 
  5. 
  

  

  rubber 
  tubing 
  and 
  clip 
  as 
  the 
  others. 
  Xear 
  the 
  bottom 
  of 
  these 
  bottles 
  

   was 
  another 
  opening, 
  to 
  which 
  a 
  similar 
  cork 
  with 
  tubing 
  and 
  clip 
  was 
  

   fitted. 
  

  

  One 
  of 
  these 
  defibrinating 
  bottles 
  and 
  one 
  of 
  the 
  store 
  bottles 
  were 
  

   filled 
  with 
  oxygen 
  gas, 
  the 
  other 
  defibrinating 
  bottle 
  and 
  the 
  other 
  store 
  

   bottle 
  with 
  carbonic 
  acid 
  gas 
  ; 
  this 
  was 
  done 
  to 
  prevent 
  the 
  blood 
  when 
  

   drawn 
  from 
  the 
  vessels 
  (artery 
  and 
  vein) 
  being 
  exposed 
  to 
  the 
  atmo- 
  

   sphere. 
  

  

  The 
  apparatus 
  being 
  thus 
  prepared, 
  blood 
  was 
  allowed 
  to 
  flow 
  through 
  

   a 
  glass 
  tube 
  (one 
  end 
  of 
  which 
  was 
  tied 
  into 
  the 
  vein, 
  the 
  other 
  into 
  the 
  

   india-rubber 
  tube) 
  from 
  the 
  jugular 
  vein 
  of 
  a 
  sheep 
  into 
  the 
  defibrina- 
  

   ting 
  bottle 
  filled 
  with 
  carbonic 
  acid 
  gas 
  ; 
  both 
  clips 
  having 
  been 
  removed 
  

   from 
  the 
  india-rubber 
  tubes, 
  the 
  gas 
  flowed 
  out 
  of 
  one 
  tube 
  as 
  the 
  blood 
  

   entered 
  at 
  the 
  other 
  : 
  during 
  this 
  process, 
  and 
  until 
  defibrination 
  had 
  

   taken 
  place, 
  the 
  bottle 
  was 
  rotated 
  upon 
  its 
  axis, 
  the 
  clips 
  being 
  re- 
  

   applied 
  as 
  soon 
  as 
  sufficient 
  blood 
  had 
  been 
  taken. 
  The 
  vein 
  was 
  then 
  

   properly 
  tied 
  and 
  the 
  carotid 
  artery 
  opened 
  ; 
  a 
  similar 
  glass 
  tube 
  was 
  

   inserted, 
  and 
  blood 
  drawn 
  from 
  it 
  into 
  the 
  defibrinating 
  bottle 
  filled 
  with 
  

   oxygen 
  gas 
  ; 
  the 
  process 
  of 
  defibrination 
  was 
  then 
  performed, 
  as 
  in 
  the 
  

   case 
  of 
  the 
  venous 
  blood, 
  and 
  the 
  clips 
  again 
  applied. 
  

  

  The 
  liquor 
  sanguinis 
  containing 
  the 
  corpuscles 
  was 
  then 
  drawn 
  off 
  

   through 
  similar 
  tubes 
  into 
  the 
  store 
  bottles 
  and 
  was 
  ready 
  for 
  testing. 
  

  

  The 
  testing-tube 
  (fig. 
  1) 
  was 
  readily 
  filled 
  from 
  these 
  store 
  bottles 
  by 
  

   inserting 
  one 
  of 
  the 
  short 
  tubes 
  (marked 
  a) 
  into 
  the 
  india-rubber 
  tubing 
  

  

  