250 



Dr. C. A. MacMunn. Researches into the [Mar. 18, 



with, the germinal vesicle, and is connected with the periphery of the 

 ovum by ramified offsets. 

 Fig. 30. Section of superficial part of ovary of a bitch pup, eighteen days old, show- 

 ing gland-like connexion of egg-tubes with the surface of the ovary. 

 str. Ovarial stroma. 



Fig. 31. From another section of the same ovary, showing more oblique connexion 

 of egg-tubes with the surface. 

 o. e. Ovarial epithelium. Stroma not represented. 

 Fig. 32. From another part, showing two other of the gland-like connexions cut 



across and obliquely. 

 Fig. 33. A very oblique connexion, cut longitudinally. 



Fig. 34. Section of superficial part of ovary of a young bitch, almost full-grown, 

 showing some of the ovarial glands (connexions of egg-tubes with 

 surface of ovary). Cut across and obliquely. 



Fig. 35. A peculiar convoluted tube from the deeper part of the same ovary, almost 

 filled with long, tapering columnar cells. 

 a. Either the same or a similar tube, cut across. 



II. " Researches into the Colouring Matters of Human Urine, 

 with an Account of the Separation of Urobilin." By C. A. 

 MacMunn; B.A., M.D. Communicated by A. GAMGEE, 

 M.D., F.R.S.. Brackenbury Professor of Practical Physio- 

 logy and Histology in Owens College, Manchester. Re- 

 ceived March 6, 1880. 



(Abstract.) 



In this paper an account of the spectroscopic and chemical characters 

 of urobilin is given. Urobilin is a pigment which has been diagnosed 

 in urine by means of the spectroscope, and which has not been hitherto 

 isolated. 



Before its discovery in urine a pigment had been obtained by Jaffe 

 by acting on human bile with nitric acid, and on dog's bile with hydro- 

 chloric acid, and subsequent treatment, which in solution gave the 

 same spectrum, and behaved in the same manner with reagents as urine 

 containing urobilin, By an examination of the bile of seventeen 

 animals, I have shown that it is present as such in the bile of all them, 

 but in greatest abundance in the bile of the mouse ; and the results are 

 described in the paper, while the spectra are shown in Chart II. 



I have also shown that human urine always gives an absorption 

 band at E, which almost always is affected in the same manner by re- 

 agents as the pigment got by Jane from bile, and that if this band is 

 not so affected another pigment called urolutein is present, which I 

 describe and have mapped in Chart I. 



I have succeeded in isolating urobilin by the following method: — 

 Having procured some urine which by preliminary spectroscopic and 



