1880.] Hydrolytic Ferments of Pancreas and Small Intestine. 399 



being required for the production of limpidity in the starch-paste con- 

 taining the most active of the three portions of the intestine, which 

 was in this, as in the former case, the region of the jejunum and 

 ileum containing the Peyer's patches. 



It is possible, as was first shown by Berthelot, to obtain a clear 

 aqueous infusion of ordinary yeast, which is capable of exercising 

 a very decided invertive action upon cane-sugar. The action of this 

 aqueous infusion is, however, feeble when compared with the inversion 

 produced by actual contact of the yeast-cells themselves. 



Reasoning from this fact, it seemed to us probable that, in the case 

 of the intestine, far more pronounced hydrolytic results might be 

 expected from acting with the tissue itself, than from merely using its 

 aqueous infusion. This was found to be the case. 



In the following experiments the different portions of the small 

 intestine, after thorough and prolonged washing, were dried rapidly 

 in a current of air at 35° C, and were divided into very fine shreds, 

 which were immersed directly in the solutions under examination. 

 The intestine was taken from a pig of eight months old, killed during 

 the period of digestion. 



The solution of cane-sugar employed contained 3*020 grms. of sugar 

 per 100 cub. centims. 



To every 100 cub. centims. of the solution were added 5 grms. of the 

 finely divided dry intestine. Temperature of digestion, 40° 0. 



All the determinations were made by the optical method. 



Comparative Action of the different portions of the Small Intestine of 

 the Pig upon Cane- Sugar. 



Portion of the small intestine. 



Percentage of cane-sugar inverted. 



After 1\ 

 hrs. at 40°. 



After 3| 

 hrs. at 40°. 



After 16 

 hrs. in the 

 cold. 



After 

 further 

 digestion 

 for 5 hrs. 

 at 45°. 



(1) Duodenum immediately be- 











low the pylorus, con- 











taining Brunner's glands 



No action. 



No action. 



No action. 



13 -0 



(2) Duodenum below the 











glands of B runner .... 



No action. 



No action. 



10 '9 



13 -0 



(3) Jejunum, not including 











any of Peyer's patches 





14 -0 



19-5 



25 1 







14-0 



19-5 



25 -1 



(5) Agminated glands of Peyer 











(Peyer's patches), cut 













9-2 



18-4 



24-6 



26-7 



