474 Mr. F. Crace-Calvert on the Action of Heat [May 11, 



Hay infusion 

 not heated. 



Heated for half 

 an hour at 

 212° Fahr. 



Heated for half 

 an hour at 

 300° Fahr. 



Heated for half 

 an hour at 

 400° Fahr. 



Heated for half 

 an hour at 

 500° Fahr. 



Fungus matter 

 was observed 

 growing on the 

 surface of the 

 fluids in two of 

 the tubes.On sub- 

 jecting the con- 

 tents of some of 

 the tubes to ex- 

 amination, from 

 20 to 25 animal- 

 cules were obser- 

 ved under each 

 field of the mi- 

 croscope. This 

 kind of life re- 

 sembled small 

 dots moving en- 

 ergetically to 

 and fro ; 1 or 2 

 ordinary vibrios 

 were also pre- 

 sent. 



No fungus mat- 

 ter was noticed 

 on the surface in 

 any of the tubes. 

 A few small black 

 vibrios present 

 in the original so- 

 lution were also 

 present in this. 



No fungus mat- 

 ter present, but 

 some of the small 

 black vibrios 

 were still pre- 

 sent, although in 

 less numbers. 



No fungus mat- 

 ter observed. The 

 fluid was filled 

 with irregular 

 masses of coa- 

 gulated matter, 

 and life had dis- 

 appeared. 



No life present. 



Gelatine Solution. — A solution of gelatine, prepared of such strength 

 that it remained liquid on cooling, was exposed for twenty-four hours to the 

 atmosphere. It was then introduced into the small tubes, and the tubes 

 sealed. The solution was made on the 4th of November, the tubes sealed 

 on the 5th, and subjected to the different temperatures on the 7th. 



The fluids were examined on the 1st of December, 1870, twenty-four days 

 after being heated. 



Gelatine solu- 

 - tion not heated. 



Gelatine solu- 

 tion heated for 

 half an hour 

 at 100° Fahr. 



Heated for half 

 an hour at 

 212° Fahr. 



Heated for half 

 an hour at 

 300° Fahr. 



Heated for half 

 an hour at 

 400° Fahr. 



There were 7 or 8 

 animalcules un- 

 der each field, 5 

 or 6 of which 

 were quite differ- 

 ent to any thing 

 observed in the 

 other fluids.They 

 had long thin bo- 

 dies, swimming 

 with a peristaltic 

 motion. 1 or 2 

 ordinary swim- 

 ming vibrios 

 were also pre- 

 sent ; but the 

 small black vi- 

 brios were ab- 

 sent. 



Life seemed to 

 have only slight- 

 ly decreased, and 

 none of the ani- 

 malcules were 

 swimming. The 

 peculiar animal- 

 cule mentioned 

 in the first co- 

 lumn appeared 

 to retain still its 

 peristaltic mo- 

 tion, but not suf- 

 ficient power to 

 move across the 

 field, a few ordi- 

 nary vibrios be- 

 ing also observed 

 moving to and 

 fro. 



A very decided 

 diminution in 

 the quantity of 

 life present was 

 noticeable. 



No life present. 



No life present. 



