On the Pathology of Cholera Asiatica. 



179 



most careful precautions. During the autopsy pieces of the intestinal 

 wall and of the other organs are placed in absolute alcohol, and all the 

 fluids through which the tissue and sections required to be passed for 

 the purpose of cutting, staining, &c, well boiled and protected from the 

 invasion of vegetable organisms by boiling and the addition of thymol. 



The micro-organism which we find in our specimens from cholera 

 cases is composed of terminal or nodal swellings connected by fila- 

 ments varying greatly in thickness, and showing no differentiation 

 into component cells. 



Fig. 1. Fig. 2. 



deep part of mucosa (Powell and Lea- 

 land oil immersion). 



Frequently also we have seen exceedingly fine filaments extending 

 from the granules, like those which form so marked a characteristic 

 of many of the Chytridiaceae. Stained with methylene -blue, the 

 granules and filaments take on the colouring matter to a fairly equal 

 degree, although with fuchsine, followed by diluted nitric acid, the 

 filaments become decolorised before the granules. The globular 

 enlargements referred to vary in size from knot-like swellings on the 

 mycelium, causing simply an inequality in the thickness of the latter, 

 to bodies as large and in some cases even larger than the nuclei of the 

 surrounding cells. In this abstract, in which we cannot give satisfac- 

 tory illustrations, we need not say more regarding the morphology of 

 the parasite than that it differs considerably in appearance in different 

 cases, always, however, within the limits included in the above 

 description, and that there are frequently present minute, round, 



