292 



Dr. G. E. C. Wood. A Method for [Feb. 27, 



action were rendered more or less refractory to subsequent infection, 

 and this suggested the possibility of its application as a means of 

 shortening the preliminary treatment which a horse must undergo 

 before it can receive the large doses of broth toxine which are usually 

 necessary for the production of antitoxine of any strength. 



The first horse (No. 1) was treated on the same general principles 

 which are adopted in immunising guinea pigs ; that is to say it receives 

 a certain quantity of the vaccinating substance and after the lapse of 

 ten or fourteen days it is subjected to the action of the microbe or 



? 



Table I. 



Horse N9 1 

 <*> 50 § 



Cfi <C C5 



Cl Qi C5 C5 



«\j Si £ Oi 





IC\J CG. OfZrUfft LUA'if 













> 









































































■* 



f 





































































































































- 























































a : 



ISO c.c. Serum Cox in. 



































































o» 





































































N 





















f 





— 





























































< 



t 







































































- 

















































v. 



















































































> 

























































c : 



//o c.c. Serum toxin 











s 



= 























































> 

















































































































































































































































































N 









































































































































& 



50c.c.3&rum toxinfurrfiker&S). 

 150 c.c broth Cox in. 

















s 









































































































































r 



































































< 





















































f\> 













































































































































































































<\> 

 





































































Ni 

















> 





















































i\, 

 Qj 



50 c.c. BroCh toxin 





















> 















































)> 

 <o 

















/ 





- 

















































o< 



<2> 



115 c.c. Broth Coxin. 



































































c* 

















s 





















































