60 Morphology of the Blastomycetes found in Carcinonrnta. 



In the nodules in lungs, liver, spleen, and kidneys, which are 

 secondary to the growths on the peritoneum, in addition to the forms 

 already described, spore-bearing forms are found. In these the capsule 

 is much thickened, the chromatin of the cell breaks up irregularly, 

 and portions are allowed to escape through dehiscences in the capsule. 

 There is no regularity in the process, no simultaneous division of the 

 cell-contents into a definite number of spores, and no simultaneous 

 shedding of the same. The spores are without capsule when they 

 escape, and are irregular in contour. They stain deeply with 

 chromatin stains, and are finely granular. This method of spore- 

 formation is specially to be noted, as it is entirely unlike the methods 

 described in the case of members of the Saccharomyces class. 



4. Tisme Reactions. — The animals used for inoculation were guinea- 

 pigs, and the inoculations were made not so much for the purpose of 

 estimating the capacity of the organism for producing cancer as for 

 that of studying the morphological characters of the latter when in the 

 tissues. 



The results following intraperitoneal injection of 1 c.c. of a culture 

 forty-eight hours' old are as follows : — The animals showed no 

 symptoms of illness ; they were killed at periods from two to six 

 weeks after the injection. On opening the abdomen, the omentum 

 and general peritoneal surface were found to be studded with nodules 

 from the size of a pea to that of a pin's head ; of the other organs, 

 nodules are visible to the naked eye in the lungs, liver, spleen, and 

 kidneys. The primary growths on the peritoneum are composed of 

 proliferated endothelial cells ; the organisms are present in consider- 

 able numbers, some within the cells, but most outside them. In many 

 of the nodules there is some attempt at the formation of a connective 

 tissue capsule, and in others the central parts are broken down. 



In the lungs, the nodules are made up of endothelial cells ; in each 

 nodule there are organisms present and usually centrally situated. 



In the liver and spleen, the nodules are very similar in appearance 

 to the primary omental growths; the origin of the cells composing 

 them is doubtful. 



In the kidney, where again the nodules are of endothelial origin, the 

 cells are derived from those lining the Malpighian corpuscles and 

 tubules. 



In no case was there any alveolar arrangement of the cells or any 

 appearance resembling the endotheliomata of man. 



