No. 560] GENETIC AL STUDIES ON (EN OTHER A 473 



The buds, 8-9 cm. long, presented a stouter cone, 4- 

 angled, and the sepal tips were less attenuate and thicker. 

 The petals were about 4 cm. long, the hypanthium was 

 shorter, and the stigma lobes, 4-6 mm. above the tips of 

 the anthers, were thicker than in the type representative 

 of the mass of the culture. The capsules, 1.8 cm. long, 

 were shorter and stouter. So many of these points of 

 difference suggest the characteristics of gigas that it was 

 not surprising to find the chromosome count to be above 

 14, the normal diploid number for CEnothera. It is diffi- 

 cult to determine the exact number, but from counts made 

 this spring at the growing points of seedlings from this 

 plant I am certain that the chromosome count is at least 

 as high as 21, the triploid number. It will be remem- 

 bered that the triploid number has been determined by 

 both Miss Lutz ('12) and Stomps ('12) for "mutants" 

 derived from lata and Lamarckiana to which Stomps has 

 given the name semi-gigas. We have then in this plant 

 (12.56a;) a variant from the parent hybrid which probably 

 corresponds closely to the "triploid mutants" of La- 

 marckiana or its derivatives. 



There will now be briefly described the F 3 generation 

 from a type 11.42/, represented by a single plant in the 

 F 2 from 10.30L& (Davis, '12a, p. 415, 11.42/). This plant, 

 about 1 m. high, was remarkable for its broad, entire, 

 much-crinkled leaves (Fig. 14) ; the flowers were medium- 

 sized (petals 2 cm. long). The contents of two capsules, 

 222 seeds, were sown, from which 117 plants were ob- 

 tained and brought to maturity (culture 12.58). The 

 mass of the rosettes consisted of broad elliptical leaves, 

 crinkled and loosely arranged. Several rosettes were 

 grandiflora-like, intergrading, however, with the mass, 

 and 8 presented a long narrow form of leaf. The culture 

 at maturity was very well graded from plants 1.6 m. high, 

 with flowers as large as those of grandiflora (petals 3.5 

 cm. long) to plants the counterpart of the F 2 parent 

 hybrid 11.42?. The foliage of the culture as a whole con- 

 tinued the progressive advance of 11.42/ as shown by 



