666 THE AMERICAN NATURALIST [Vol. XLYII 



Alcoholic male No. 43 has been mated 10 times. Three 

 of the matings gave negative results, two by alcoholic 

 females and one by a normal. One stillborn litter re- 

 sulted from a mating with an alcoholic female. Six living 

 litters came from the 10 matings. The young in 3 of 

 these litters died soon after birth and 3 litters survived. 



Alcoholic male No. 45 has now been treated about one 

 and one half years and has been mated 9 times. One 

 mating with a normal female gave a negative result. One 

 mating with a normal female gave a stillborn litter. 

 Seven living litters were produced, 5 of which survived, 

 2 from alcoholic females and 3 from normal females. 

 The data in this case appear slightly better than in the 

 foregoing but this is due to the fact that only a few 

 matings have been made and most of these during the 

 early stages of the treatment, when the effects are not so 

 pronounced. 



All of the other alcoholized males in the experiments 

 show comparable records. 



A reference to Table I shows that with three excep- 

 tions 35 matings of normal males with normal females 

 gave living litters containing in all 60 individuals, only 4 

 of which failed to survive. This record stands in striking 

 contrast to the data recorded above from the 4 alcoholic 

 males, and it shows convincingly that the alcoholic treat- 

 ment has affected the germ cells of these males so that 

 they are no longer capable of producing entirely normal 

 offspring even though they be mated with normal females. 



The outcome of the successive matings of fifteen differ- 

 ent females is tabulated in Table II. The varying ways 

 in which the same individual has responded in different 

 matings is noticeable. Number 15, a normal female, 

 shows an instructive record. Mated with alcoholic male 

 No. 6 she gave two stillborn young ; mated with alcoholic 

 male No. 5 a negative result; remated with No. 5, two 

 young were born and both died of convulsions during the 

 fourth week; then mated with a normal male a normal 

 vigorous offspring was produced ; mated again with alco- 



