1901.] Spur 



-ge (Euphorbia hiberna, L.) on Salmonoicl Fishes. 57 



Strength and 

 nature of 

 solution. 



0"2per cent, of 

 "fresh, ex- 

 tract " from 

 stem and 

 leaves 



0*2 per cent, of 

 " fresh ex- 

 tract " from 

 roots 



'2 per cent, 

 of emulsion 

 minus Tola- 

 tile solid 



'2 per cent, 

 of emulsion 

 minus vola- 

 tile solid. 4 

 weeks later 



Solution of 

 volatile solid 



1 per cent, of 

 " i'resh ex- 

 tract" of 

 Spurge 



100 per cent, 

 of do. 



100 per cent, 

 of do. 



100 per cent, 

 of do. 



100 per cent, 

 of do. 



20 per cent, of 

 do. 



"1 per cent, of 

 tannic acid 



Period 

 within which 

 it is fatal. 



2 to 4 hours 



1 to 3 hours 



40 minutes 

 to 1 hour 



1 to 2 hours 



1 per cent, 

 do. 



of 



Animal 



Mode of 



experi- 



applica- 



mented on. 



tion. 



Trout 



Animals 





immersed 





in solu- 





tion 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 



Gastroc- 



Direct irri- 



nemius 



gation 



of pithed 





frogs 





Do. 



Do. 



Do. 



Injected 





into 





lymph 





space 



Heart of 



Direct irri- 



pithed 



gation, 



toads 



and in- 





jection 



Lung of 



Direct ir- 



pithed 



rigation 



frog 





Do. 



Do. 



Do. 



Do. 



Do. 



Do. 



Notes. 



this way is stronger than 

 the fresh extract. Thus 

 0*2 per cent, of this is 

 equivalent to about 0*5 

 per cent, of fresh ex- 

 tract. 



Death did not result, 

 although animals dis- 

 played excitement. Per- 

 haps quantity too small. 



No effect produced. The 

 .responses given did not 

 'differ from those of 



normal frog. 



No effect produced. 

 Heart's action remained 

 normal. 



The lung contracted vis- 

 ibly. The capillaries 

 were constricted and a 

 condition of stasis set 

 up in larger vessels, all 

 within a few minutes. 



The effect produced was 

 precisely similar to that 

 described as due to 

 the Spurge-extract, the 

 stronger solutions being 

 more rapid in their 

 action. 



