58 



Dr. H. M. Kyle. On the Action of the [June 25 r 



Strength and 

 nature of 

 solution. 



Period 

 within which 

 it is fatal. 



100 per cent, 

 of " fresh ex- 

 tract." 



20 per cent, of 

 do. 



2 per cent, of 

 do. (approx.) 



Do. 



2 per cent, of 

 do. (approx.) 



01 per cent. 

 tannic acid 



0'02 per cent, 

 tannic acid 



Finely pow- 

 dered starch 

 in state of 

 suspension in 

 water 



Emulsion of 

 resin and 

 mucilage in 

 water 



001 per cent, 

 tannic acid 

 and starch 

 and aleurone 



*5 c.c. of 

 " fresh ex- 

 tract " 



Animal 

 experi- 

 mented on. 



Pithed 

 frog's 

 mesen- 

 tery 



Do. 

 Frog 



Frog 

 pithed 



I Frog 

 pithed 



Mode of 

 applica- 

 tion. 



5 to 6 hours 



2.V hours 



Trout 



Do. 



Do. 



2i h( 



12 hours 



Do. 



Do. 



Do. 



Direct ir- 

 rigation 



Immersed 

 in solu- 

 tion, but 

 so that 

 head 

 was free 



Injection 



Injection 



Immersed 

 in solu- 

 tion 



Do. 



Do. 



Do. 



Injected 

 into ab- 

 domen 



Effect as on lung, but 

 seen to be local. Circu- 

 lation was maintained 

 in neighbouring vessels 

 not affected by the ex. 

 tract. 



Lived thus for 7 days. 



Reflex time for response 

 to stimuli taken before 

 and after injection, 

 greater in latter case. 



A stronger electrical sti- 

 mulus required to ob- 

 tain a response, under 

 the influence of the 

 Spurge - extract than 

 under normal condi- 

 tions. 



Fish quiescent, no para- 

 lysis apparent, breath- 

 ing irregular after some 

 time, gill-covers a little 

 strained but not greatly. 



Post-mortem examination 

 show r ed that great 

 sloughing of the epithe- 

 lium of the gills had 

 taken place, more es- 

 pecially in the stronger 

 solution. 



Animal immersed for 5 

 hours without showing 

 any signs of discomfort. 

 Animal took food in 

 solution. A fine powder, 

 tiierefore, is not harm- 

 ful in itself. 



IVo effect. 



Eapidity of action ap- 

 parently increased. 



The first specimen in- 

 jected was much larger 

 than other two — the 

 third small and thin. 

 Symptoms similar. At 

 first no signs of change. 



