354 



Dr. P. F. Frankknd aud Mr. J. J. Fox. [June 20 



I. 2 "8.505 grams Ba-salts giving 91*66 per cent. BaS0 4 . 

 II. 3-2630 „ „ 93-17 



III. -5360 gram Ba-salts. 



6 -6495 



Pare Ba-acetate yields 91'37 per cent. BaS0 4 . 

 Pure Ba-formate yields 102*64 per cent, BaS0 4 . 



Thus, whilst the first fraction was practically pure barium acetate, 

 the second fraction contained a notable proportion of barium formate, 

 the composition of this fraction, as calculated by the aid of the 

 formula given on p. 351, being — 



Barium acetate 2*7419 grams 



„ formate 0*5211 „ 



3*2630 „ 



The third fraction doubtless contained an even larger proportion of 

 barium formate, but the barium determination was unfortunately 

 lost. 



It may, however, be taken that the volatile acids produced in this 

 fermentation consisted of about 2*75 grams acetic and 32 gram 

 formic acid. 



Fixed Acids. — After distilling off the volatile acids as above, the 

 residue containing any fixed acids, together with calcium chloride 

 and unaltered glycerol, was treated with sodium carbonate to precipi- 

 tate the calcium, which was filtered off ; the filtrate was evaporated 

 to dryness and extracted with a mixture consisting of 5 vols, of 

 alcohol and 1 vol. of ether. Such a mixture, whilst dissolving 

 glycerol, does not dissolve either sodium lactate or succinate. From 

 the ether-alcohol extract 22*8615 grams of unaltered glycerol* were 

 recovered. The residue, insoluble in ether-alcohol, was treated with 

 strong boiling alcohol to extract any sodium lactate, but none was 

 obtained, the residue remaining after treatment with alcohol was 

 dissolved in water, acidulated with hydrochloric acid, and repeatedly 

 shaken up with ether ; from this ethereal extract 0*079 gram of suc- 

 cinic acid was obtained. 



The results show that only a part of the glycerol had undergone 

 fermentation (nearly one half having been recovered), whilst the 

 principal products were alcohol and acetic acid, together with a small 

 quantity of formic acid and traces of succinic acid. 



Fermentation of Glycerol No. 2. 



In this experiment the fermenting liquid was allowed to remain in 

 the incubator at 37 — 39° C. for five months before examination. 

 * This glycerol yielded on incineration only 0*85 per cent, of ash. 



