550 



Drs. T. L. Brunton and A. Macfadyen. 



2nd day. Starch has lost its opalescence. Iodine gives a blue 

 colour. 



3rd day. I dine gives a red colour. 

 5th day. No reaction with iodine. 



6th day. Was tested for a reducing sugar. The reactions were 

 as follows : — 



(] .) Iodine. — No reaction. 



(2.) Caustic soda. — On gently boiling fluid becomes yellow. 

 (3.) Cupric sulphate and caustic soda. — A yellow precipitate on 

 boiling. 



(4.) Fehling's reagent. — A red precipitate. 



(5.) Barfoed's reagent. — ]S"o reaction on gently heating. 



(Barfoed's Solution : — One part of neutral acetate of copper dissolved 

 in 15 parts of water, and then to 200 c.c, 5 c.c. of acetic acid 

 (38 per cent.) added.) 



The control starch gave blue colour with iodine, but none of the 

 above reactions. 



B. Putrefactive micrococcus — 

 Results were negative. 



C. Koch's spirillum. 

 Tinkler's spirillum : — 



The same starch solution was used, but a few drops of meat broth 

 were added in each case. The usual control experiments were 

 made : — 



3rd day. Starch has lo; t its opalescence. Iodine strikes a blue 

 colour. 



4th day. Iodine gives a violet colour. 

 5th day. Iodine gives red reaction. 

 7th day. Iodine. — Red. 



Caustic soda. — Yellow on boiling. 



Cupric sulphate and caustic soda. — No reduction. 



Fehling's solution. — No reduction. On previous addi- 

 tion of H 2 S0 4 a slight reduction. 



Barfoed's reagent. — No reduction. 



Control starch. — Iodine strikes blue. 



From these experiments the following conclusions may be 



drawn : — 



1. The putrefactive micrococcus did not grow on the carbohydrate 

 soil, and so we are left in doubt as to its diastatic action. 



2. The scurf bacillus and Welford bacillus were both capable of 

 cultivation, and evinced a marked diastatic action, in addition to 

 their peptonising power. The failure of the iodine test, and the 



