164 Nuclei in the Fertilised Embryo-sac of Lilium Martagon. 



after fixing, to make sure that it really contained fertilised embryo-sacs. 

 Fourteen sections were kept, all of them stained with methyl green 

 and acid fuchsin. As this, though a brilliant, is rather a diffuse stain, 

 I have lately re-stained eight of the preparations with Renault's hema- 

 toxylin and. eosin, which gives more precise results. 



None of these preparations show the vermiform nuclei free in the 

 embryo-sac. In every case conjugation has already taken place ; the 

 male nucleus is applied to the female nucleus in the micropylar end of 

 the embryo-sac, and the second generative nucleus is applied to both 

 polar nuclei. In one case only the two polar nuclei are not in contact. 

 The much elongated " antherozoid " unites them like a bridge, one end 

 in contact with the lower, the other end coiled round the upper 

 nucleus (fig. 1, v.ri). 



nvcropyle. 



Excluding all doubtful cases, eight embryo-sacs show the male and 

 female nuclei not } et fused but in contact. In six of these the male 

 nucleus is more or less elongated. It may be distinctly coiled (fig. 1,-g.ri), 

 or merely horse-shoe or kidney-shaped, and commonly lies on the upper 



