and Medicinal Reagents on Bacillus tuberculosis. oil 



Pasteur's solution (without sugar). 



Beef solution, 1 ounce of meat to 2 ounces of water. 



„ \ ounce of meat to 2 ounces of water. 



Pork broth (Klein). 



Also distilled water and the subjoined medicinal agents in solution 

 were mixed, in generally equal proportions, with the sputum, and 

 kept at the same temperature as above. 



Solutions of quinine in strengths varying from 2 grs. to the ounce 

 to 10 grs. to the ounce. 

 „ ,, arsenious acid, \ gr. to the ounce and 1 gr. to the 



ounce. 



„ ,, boracic acid, 1 part in 30 and 1 part in 15. 



,, ,, iodine, 1 part in 12. 



,, ,, perchloride of mercury, 1 gr. to the ounce. 



The results were as follows : — 



Syrup. — Cultivation was carried on for eight days. The tubercle 

 bacilli were found in diminishing numbers in the first forty-eight 

 hours; they afterwards gradually disappeared, and during the last 

 three days none were detected. On the first day they were of fair 

 length, and some groups were noted. 



Hay Infusion. — Four days' cultivation. Bacilli were present in the 

 first twenty-four hours, but afterwards disappeared entirely. 



Pasteur's Solution. — Eight days' cultivation. Bacilli diminished in 

 numbers at first and then remained stationary for some days. They 

 showed signs of increase on the eighth day. 



Beef Solution. — Two strengths were experimented on during seven 

 and eight days. In the stronger solution the bacilli increased 

 enormously, both in numbers and in length, and in some fields of the 

 microscope it was impossible to count them. Groups of five and ten 

 were not uncommon, but those of greatest length appeared isolated. 

 Some were long and slender, without cross markings, others shorter 

 and slightly broader with well-marked spores. The bacilli were most 

 abundant in the neighbourhood of the meat debris, round which they 

 swarmed like ants on sugar. As the solution became decomposed, 

 the number of bacilli decreased, but about the seventh or eighth day, 

 they were seen increasing by division. The Bacterium termo and other 

 bacilli were also present. A weaker solution of beef gave the same 

 results, though to a less marked extent. 



Boric Broth. — Four days' cultivation. The results were different, 

 for the bacilli did not increase in numbers, but rather declined, 

 though they did not totally disappear from the solution. 



Distilled Water. — Two to three days. The sputum of three 

 different patients was mixed with distilled water, and in each case no 

 growth or multiplication of bacilli was observed. 



Sulphate of Quinine. — A large number of experiments were made 



