On Trichophyton tonsurans (the Fungus of Ringworm), 239 



tion occur, so far as a curdled condition is evidence of that change. 

 A thin stratum of clear fluid in one case appeared on the surface of 

 the milk, but to a much less extent than in ordinary curdling. 



4. Aqueous Humour. — Two experiments only were made with aqueous 

 humour, the cells charged with it being placed in the incubator. In one 

 after five days there was no growth of the ringworm spores ; in the 

 other, during forty-eight hours, a bulging had taken place from some 

 of the spores, evidently the beginning of growth. On account of the 

 greater convenience in getting large quantities of fluid vitreous 

 humour was used instead of aqueous humour in subsequent experi- 

 ments. 



5. Tap Water. — A ringworm hair, moistened with tap water, was 

 placed in the incubator. During nine days there was no growth. 



6. Carrot Infusion. — One cell was prepared with carrot infusion. 

 During four days no growth took place. 



7. Salt Solution. — Strength 075 per cent. — A cell thus prepared 

 was placed in the incubator for five days : no growth. Hairs were 

 floated on the solution in a glass in the incubator for six days without 

 growth of trichophyton spores. 



8. Turnip Infusion. — Bread, soaked in turnip infusion, was placed 

 in a box and ringworm hairs placed on the bread at different points. 

 The box was covered and placed on the top of the incubator, where 

 the heat was less than inside. After four days the bread was found 

 covered with fungi. 



The ringworm hairs were picked out, macerated in potash, and 

 examined. All the spores were round, and there was no appearance 

 on the sides of the hairs of bulging or early mycelium formation. 



Ringworm hairs were floated on pure turnip infusion in protected 

 glasses, and placed in the incubator for five days. They were then 

 examined. The spores observed in the hairs were all round, and there 

 was no growth from the sides. Another experiment of the same kind 

 in which the hairs were examined after four days, showed a like 

 negative result. 



In another similar experiment the hairs were found after eight days 

 imbedded in fungus, Penicillium glaucum. The hairs, when examined, 

 were seen to be full of spores, which had not sprouted from the sides 

 in the least degree ; those which were lying on the extreme edge of 

 the hair being found round and unchanged. 



In two cells charged with diseased hairs, minute morsels of bread and 

 turnip infusion, and placed in the incubator, no ringworm growth took 

 place during four days ; and another experiment, in. which the hairs 

 were floated on the infusion and kept in the incubator for seven days, 

 also showed a negative result. 



The attempts to grow trichophyton in turnip infusion thus all 

 failed. 



