240 



Dr. G. Thin. 



9. Egg Albumen. — Seven attempted cultivations with egg albumen 

 in the incubator, and one at room temperature, also one with acid 

 albumen in the incubator, conducted in the same manner as those 

 already described, all failed. 



10. Egg Albumen and Potash. — Four attempted cultivations with 

 albumen, to which a small proportion of solution of potash had been 

 added, also failed. In two of them the hairs were floated on the surface 

 of the fluid in glasses. placed in the incubator; in the other two the 

 hairs had been sunk to the bottom. 



11. Vitreous Humour and Potash. — Three cultivation experiments 

 with vitreous humour and potash, similarly conducted, in one of which 

 the ringworm hairs were floated on the top, whilst in the other two 

 they were sunk to the bottom, also failed. 



12. Vitreous Humour. — As vitreous humour is the only fluid with 

 which I have succeeded in cultivating trichophyton, and as, even 

 with this fluid, special conditions are necessary, it is desirable to 

 describe the experiments and mode of procedure in detail. 



It will be convenient to divide them into — 



1. Cultivation in cells at incubator temperature ; no growth taking 

 place. 



No. of days 



'No. of cells. in incubator. Kemarks. 



1 2 



3 4 



4 5 



1 6 



3 7 



6 8 



5 9 



1 10 



1 11 



2 12 



5 . . No. of days in incubator 



— not noted. 



32 



The cells were, during these periods, examined daily, and imme- 

 diately afterwards put back again into the incubator. Those regard- 

 ing which it is remarked that the time was not noted are the cells 

 which were used in the earlier experiments. They were kept a long 

 time, and frequently examined, the date on which they were finally 

 taken out not being noted. 



In the earlier of these experiments, the hairs used had been extracted 

 for some time from the patients, and had been kept folded in paper. 

 This may possibly have had something to do with the negative results. 



