192 Dr. A. Ransome, On certain Media for 



Table IV. 



No. 





Date of 

 inocula- 

 tion. 



Periods of incubation and growth (in 

 incubator at 35° C). 



a weeks. 





o weeks. 



12 weeks and 

 upwards. 





Media : — Sterilised 















potato, and the va- 















pours as above 













1 



From cellar in pure 

















April 3 



X 



XXX 



X X X X 



X X X X 



2 







X 



X X 



X X 



Feeble 



3 



inipux'G cgI-Ieu* in clciy 



10 







X X 



XXX 



4 





)> »> 







X X 



XXX 



5 



HeaLthy breath .... 



» 9> 





X 



X X ' 



X X 



6 



Ditto 



J> >> 





X X 



X X 



XXX 



7 



Phthisical breath . . . 



3 





X 



X X 



XXX 



In the next series of trials, it was decided to use as the material 

 bases some no n -nitrogenous substance, and attempts were made to 

 employ pieces of wood, cork, cotton-woo), and fine spun glass, the 

 last named at the suggestion of a distinguished bacceriologist. None 

 of these bases were found to be satisfactory ; and at length it was 

 determined to use a particularly pure " filter-paper," manufactured 

 by Messrs. Schleicher and Schiill, from which even the salts had 

 been extracted by washing with hydrochloric and hydrofluoric 

 acids.* This paper was folded in a convenient form, sterilised, 

 inserted in the test-tubes, and charged with the several organic fluids, 

 to which, as before, 6 per cent, of pure glycerine had been added. 

 It was then inoculated, stoppered as before, and in the first trials 

 these tubes were placed in the incubator at the usual temperature of 

 35° C. 



The results are shown on Table V. 



It will be seen that some degree of success was attained in twelve 

 out of fifteen specimens of the organic fluids. The degree of growth 

 was also much the same as in the previous series, though perhaps 

 slightly less vigorous. 



* Each of these filter-papers, analysed for me by the Kjeldahl process, by Sir H. 

 Roscoe's assistant, was found to contain only * 1 milligram of nitrogen. 



