188 



Profs, Percy Frankland and Marshall Ward. 



Tabular Description of Bacteria isolated from the Waters of the Rivers Thames and Lee, and from Deep Wells in 



the Chalk (Grace and Percy Frankland) — continued. 



Growth and 

 action in nitrate 

 soluiion. 



No reduction of 

 the nitrate 

 takes place. 



Powerfully re- 

 duces nitrates 

 to nitrites. 



Potato cultiva- 

 tions. 



Produces a 

 moist and 

 shining choco- 

 late coloured 

 expansion, 

 which extends 

 over the whole 

 surface. 



It is unable to 

 grow on pota- 

 toes. 



Broth culti- 

 vations. 



Renders the 

 liquid very 

 turbid and 

 viscid. Later 

 a thin green- 

 white pellicle 

 is formed. 



The liquid 

 is rendered 

 slightly tur- 

 bid, and later 

 on a violet 

 deposit is pro- 

 duced. 



Agar agar 

 cultivations. 



The whole sur- 

 face rapidly 

 assumes a 

 green appear- 

 ance, and a 

 smooth green- 

 white expan- 

 sion is pro- 

 duced. 



Forms a beauti- 

 ful deep violet 

 coloured ex- 

 pansion, which 

 spreads over 

 the whole sur- 

 face. 



Gelatine-tube cultiva- 

 tions. 



Causes rapid liquefac- 

 tion of the gelatine, 

 producing green fluor- 

 escence throughout 

 the contents of the 

 tube, which becomes 

 excessively viscid. 



Liquefaction taites place 

 in the form of a 

 funnel, the liquid be- 

 comes turbid and at 

 the bottom of the 

 funnel the violet pig- 

 ment collects. 



Appearance in gelatine-plate 

 culture. 



In the depth the colonies 

 appear smooth - rimmed; 

 later, when liquefaction 

 commences, the periphery 

 exhibits fine hair-like ex- 

 tensions. The gelatine is 

 rapidly liquefied, and each 

 colony is surrounded by a 

 green fluorescent zone. 



In the depth the colonies 

 appear irregular in con- 

 tour, which increases with 

 the age of the colony. It 

 forms a circular depres- 

 sion in the gelatine, and 

 later the characteristic 

 violet p*'gment makes its 

 appearance. Liquefaction 

 does not take place 

 rapidly. 



Microscopic appearance. 



Short bacillus with 

 rounded ends, from 

 1*5 to 2 ju. long, about 

 three or four times as 

 long as broad. Occurs 

 usually in pairs. No 

 spores observed. It is 

 exceedingly motile. 



Short bacillus, varying 

 in size, about l - 7 /* in 

 length and 0*8 ;u in 

 width. It generally 

 occurs in pairs. When 

 grown on agar it as- 

 sumes a far more 

 slender appearance, 

 and also gives rise to 

 short threads. Spore 

 formation was ob- 

 served. It is motile 

 but restricted chiefly 

 to vibratory and ro- 

 tatory movements. 



Name of micro- 

 organisms 

 and where found. 



No. 8.— Bacillus 

 viscosus. 

 Found very fre- 

 quently in unfil- 

 tered river water 

 of the Thames and 

 Lee, also occasion- 

 ally in the same 

 water after filtra- 

 tion, whilst it is 

 only rarely found 

 in deep-well water. 



No. 9.— Bacillus 

 viola ecus. 

 Originally ft und in 

 River Spree water, 

 but found by us 

 also in the Rivers 

 Thames and Lee, 

 also in deep-well 

 water from the 

 chalk. 



