Report on the Bacteriology of Water. 215 



in a corresponding experiment made on the same day (October 8) 

 with the porcelain- filtered water (which was also orginally infected 

 with about the same number of anthrax germs as the unfiltered 

 water), it will be seen that the numbers have not diminished to 

 nearly the same extent in the porcelain- filtered water as in the 

 unsterilised water ; in fact, in the porcelain-filtered water they have 

 undergone hardly any reduction at all. 



The following experiments were conducted in order to investigate 

 the virulence of this unfiltered water in which plate cultivation had 

 revealed the presence of living anthrax germs : — 



Animal Experiment No. 21. — On October 23, 1892, 1 c.c. of the 

 water in flask "II, unfiltered Thames water (Second Series), in- 

 fected with anthrax, March 25, 1892," was subcutaneously injected 

 into a white mouse. The mouse died within 4 days 16 hours, anthrax 

 bacilli being duly found in the spleen. 



Animal Experiment No. 26. — On October 29, 1892, 1 c.c. of the 

 water in flask "21, unfiltered Thames water (Second Series), in- 

 fected with anthrax, March 25, 1892," was subcutaneously injected 

 into a white mouse. The mouse is still alive (November 14, 1892) 

 16 days after the operation, and therefore out of danger of anthrax. 



Animal Experiment No. 25. — On October 29, 1892, 1 c.c. of water 

 from the flask " 3 R, unfiltered Thames water (Second Series), in- 

 fected with anthrax, March 25, 1892," was subcutaneously injected 

 into a white mouse. The mouse died within 5 days 16 hours ; the 

 body exhibited extensive oedema, and the spleen was found full of 

 anthrax bacilli, which was further confirmed by the gelatine cultiva- 

 tions prepared from that organ. 



Animal Experiment No. 33. — This was performed on November 5, 

 1892, and was a repetition of Experiment No. 26. The mouse died 

 within 2 days 4 hours. The spleen was much enlarged and found to 

 contain anthrax bacilli, the characteristic growth being obtained in 

 gelatine cultivations from the same organ. 



Animal Experiment No. 34. — On November 5, 1892, 1 c.c. of water 

 from the flask "2R, unfiltered Thames water (Second Series), in- 

 fected with anthrax, March 25, 1892," was subcutaneously injected 

 into a white mouse. The mouse died within 6 days 16 hours ; the 

 body exhibited much oedema ; the spleen was considerably enlarged, 

 and an abundance of anthrax bacilli were found in it. 



We may conveniently also refer at this point to experiments made 

 with some flasks resembling the above in all respects excepting that 

 from July 23, 1892, onwards, when they were taken out of the in- 

 cubator and refrigerator respectively, they had been exposed to 

 diffused daylight, whilst the above flasks had been kept in the dark 

 throughout. The results of the plate cultivations from these day- 

 light flasks, which do not call for any special comment beyond that 



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