Report on the Bacteriology of Water. 



225 



Animal Experiment No. 7. — On October 12, 1892, 1 c.c. of water 

 from the flask " 2 I, Thames water, porcelain-filtered (Second Series), 

 infected with anthrax, March 25, 1892," was subcutaneously injected 

 into a white monse. The mouse died within 2 days 5 honrs ; the 

 body exhibited much oedema, bat the spleen was very small ; anthrax 

 bacilli were found in the latter, both with the microscope and by 

 cultivation in gelatine. t 



From these experiments it is evident, therefore, that the sterile 

 Thames waters (Second Series) were still virulent nearly 7 months after 

 their infection with anthrax, and the virulence as measured by the 

 rapidity of their lethal effect was noticeably greater than with the corre- 

 sponding waters of the First Series. 



III. Experiments on the Vitality and Virulence of the Bacillus 

 anthracis and its Spores in Loch Katrine Water. 



Experiments were made on the same lines with the water supplied 

 to Glasgow from Loch Katrine. 



The water was collected personally by myself, on July 6, 1892, at 

 the Anderson's College, Glasgow; some of the water was drawn 

 directly into sterile bottles, and submitted to ordinary plate cultiva- 

 tion within a few hours of its collection. The plates, after three 

 days' incubation at 18 — 20° C, yielded 74 colonies per 1 c.c. of water. 



As this is, as far as I am aware, the first record of the plate 

 cultivation of such moorland water, I take this opportunity of also 

 referring to the results obtained by me, for another purpose, in 

 the plate cultivation of a number of samples of that portion of the 

 Dundee water supply which is derived from the Loch of Lintrathen, 

 a very similar source to Loch Katrine. All the samples were taken 

 during the months of June and July, and one in October, during the 

 present year, and were submitted to cultivation in about one hour 

 from the time of collection. The results were as follows : — 





No. of days the plates 



No. of colonies ob- 



Date of 



were incubated at 



tained from 1 c.c. 



collection. 



18—20° C. 



of water. 



22.6.92 



4 



110 



23.6.92 



4 



149 



30.6.92 



3 



290 



2.7.92 



3 



94 



4.7.92 



3 



114 



11.7.92 



3 



279 



17.7.92 



3 



177 



21.7.92 



3 



77 



29.7.92 



3 



155 



18.10.92 



3 



260 



