Report on the Bacteriology of Water. 



237 



water from the flask "IT, Loch Katrine, unaltered, infected with 

 anthrax, July 8, 1892," was subcutaneously injected into a white 

 mouse. The mouse is still alive (November 10, 1892), or 25 days 

 after the operation. 



Animal Experiment No. 22. — On October 23, 1892, 1 c.c. of water 

 from the flask "21, Loch Katrine, unfiltered, infected with anthrax, 

 July 8, 1892," was subcutaneously injected into a white mouse. The 

 mouse is still alive (November 10, 1892), or 18 days after the opera- 

 tion. 



Animal Experiment No. 19. — On October 21, 1892, 1 c.c. of water 

 from the flask " 1 R, Loch Katrine, unfiltered, infected with anthrax, 

 July 8, 1892," was subcutaneously injected into a white mouse. The 

 mouse died within 2 days 15 hours ; the body exhibited extensive 

 oedema ; the spleen was much enlarged, and was found full of anthrax 

 bacilli, the characteristic growth being obtained in gelatine cultivation. 



Animal Experiment No. 23. — On October 23, 1892, 1 c.c. of water 

 from the flask " 2 R, Loch Katrine, unfiltered, infected with anthrax, 

 July 8, 1892," was subcutaneously injected into a white mouse. The 

 mouse died within 2 days 16 hours, and, although no bacilli could be 

 microscopically detected in the spleen, their presence was revealed by 

 gelatine cultivations made from that organ, thus leaving no doubt that 

 the animal succumbed to anthrax. 



Thus, the experiments made on mice with the unsterilised Loch Katrine 

 water are in precise harmony with the results obtained by plate cultiva- 

 tion ; the two incubator flasks failed to hill, whilst the two corresponding 

 refrigerator flaslcs were fatal to the mice, into which they were injected 

 in the same quantity. 



The same striking contrast was likewise obtained in the case of 

 two similar flasks, which had, however, been exposed to daylight 

 since July 23, 1892. Thus— 



Animal Experiment No. 12. — On October 17, 1892, 1 c.c. of water 

 from the flask " 3 1, Loch Katrine, unfiltered, infected with anthrax, 

 July 8, 1892, exposed to daylight since July 23, 1892," was sub- 

 cutaneously injected into a white mouse. The mouse is still alive 

 (November 10, 1892), or 18 days since the operation. 



Animal Experiment No. 13. — On October 17, 1892, 1 c.c. of water 

 from the flask " 3R, Loch Katrine, unfiltered, infected with anthrax, 

 July 8, 1892, exposed to daylight since July 23, 1892," was sub- 

 cutaneously injected into a white mouse. The mouse died within 

 2 days 18 hours ; the body exhibited much oedema ; the spleen was 

 slightly enlarged, and anthrax bacilli were discovered in the latter, 

 both by the microscope and by cultivation in gelatine. 



Thus, in the case of the flaslcs subsequently exposed to daylight, also, 

 the incubator flash proved harmless, and the refrigerator flash fatal, to 

 the mice, into which they were respectively injected in equal quantity. 



