238 Profs. Percy Franklancl aud Marshall Ward. 



It was not, however, to be forthwith concluded, that the anthrax 

 germs in these incubator flasks were necessarily extinct, and the 

 endeavour was made, as in previous cases described above, to revive 

 them, by the addition of broth to the waters. On October 29, 

 1892, therefore, 5 c.c. of sterile broth were accordingly added to each 

 of the flasks " 1 1," and " 2 I," which were then placed in an incubator 

 at 37° 0., after which the following two experiments were per- 

 formed : — 



Animal Experiment No. 28. — On October 31, 1892, 0'5 c.c. of the 

 water from the flask (to which broth had been added on October 29, 

 1892) " 1 1, Loch Katrine, unfiltered, infected with anthrax, July 8, 

 1892," was subcutaneously injected into a white mouse. The mouse 

 is still alive (November 14, 1892), or 14 days after the operation, 

 and, therefore, out of danger of succumbing to anthrax. 



Animal Experiment No. 29. — On October 31, 1892, 0*5 c.c. of the 

 water from the flask (to which broth had been added on October 29, 

 1892) " 2 I, Loch Katrine, unfiltered, infected with anthrax, July 8, 

 1892," was subcutaneously injected into a white mouse. The mouse 

 died within 2 days 19 hours, anthrax bacilli being found in the 

 spleen, and the characteristic growth obtained on gelatine cultiva- 

 tion. 



Thus, in the case of flask " 1 1," the anthrax germs were extinct 

 and could not be revived with broth ; in the case of " 2 I," however, 

 the addition of broth restored the virulence, so that some few anthrax 

 germs must still have been alive in this flask. 



Turning, in the next instance, to Table XIII, we find that on the 

 day of infection (July 8, 1892) the porcelain-filtered Loch Katrine 

 water yielded about 4000 colonies per cubic centimetre, whilst four 

 days later (July 12, 1892) the number had risen to about 8000 in the 

 flask kept at 18 — 20° C, whilst the refrigerator flask exhibited only a 

 slight increase on the original number, a similar difference being 

 observable again on the tenth day (July 18, 1892). From July 23, 

 1892, the temperature of the refrigerator was permitted to follow 

 that of the room, and on October 8, 11, and 12, 1892, these refrigerator 

 flasks yielded 6000 — 8000 anthrax colonies, whilst the incubator ones 

 had fallen again to the original number of about 4000 per cubic 

 centimetre. 



Again, in the case of the flasks which had been exposed to the day- 

 light from July 23, 1892, onwards, it was found, on October 12, 

 1892, that the refrigerator flask contained about 10,000, the incubator 

 flask only 6000, anthrax germs. 



It will be seen, therefore, that although there is evidence of the 

 numbers being longer maintained at the low than at the high tem- 

 perature, the contrast between the two is enormously less marked than 

 in the case of the unsterilised Loch Katrine water. 



