258 



Profs. Percy Frankland and Marshall Ward. 



The anthrax employed was a very virulent one, and known as 

 " Edinburgh Cow A," grown on agar for thirty hoars at 30° C. 

 Eight flasks were used, and divided into four pairs ; each flask re- 

 ceived 2-5 c.o. of the water to be examined, and a large charge of 

 anthrax — 1 c.c. of the infecting fluid, which contained chiefly, if not 

 entirely, bacilli. 



Two flasks were charged with the Thames water in its crnde state, 

 and not infected at all. 



Two were charged similarly with the crude water forthwith infected 

 with anthrax. 



Two were charged with the Thames water forthwith filtered 

 through porcelain, and at once infected. 



Two were charged with the boiled Thames water infected at once 

 on cooling. 



All stood at 20° C. in the dark. 



In order to meet any such objection as that the original water 

 possibly contained the spores of anthrax, we proceeded as follows : — 



On June 27, the flasks having stood for seven days, we took samples 

 of the original raw (non-infected) Thames water, and heated them 

 at 60° C. for twenty-four hours. Plates made from this gave no signs 

 of anthrax colonies. We also inoculated a guinea-pig with 1 c.c. of 

 the raw water (not sterilised) injected into the peritoneum; this 

 animal lived uninjured, whereas a guinea-pig inoculated with 1 c.c. 

 of the raw water infected with anthrax died in thirty- six hours, and 

 cover glass preparations and cultures made from the organs proved 

 that it died normally of anthrax. 



