21)0 



Profs. Percy Frankland and Marshall Ward. 



Table a shows very clearly how the anthrax rapidly falls in 

 quantity during the first three days, whereas the normal aquatic 

 flora takes the lead and rnns through the usual phases of rapid rise 

 to a maximum and then an eventual fall. I have not included their 

 further behaviour here, however, because it was impossible to trace 

 the anthrax any longer on the plates. 



On June 27, we heated a sample of the water — having then stood 

 peven days at 20° C — at 60° C. for twenty-four hours, and made a 

 series of plates from it. With the exception of one or two water 

 organisms on one of the plates, we obtained beautifully pure cultures 

 of anthrax, proving beyoud doubt that spores had been introduced or 

 formed in the flasks. This was confirmed by inoculating a guinea-pig. 



