Report on the Bacteriology of Water. 



293 



-A 



A, glass cylinder; B, Chamberland filter ; C, sterilised cotton wool ; D, sterilised 

 paper cap tied over the cotton wool ; E, Bacillus fluoresceins liquefaciens ; 

 F. Bacillus anthracis. 



no spores — while anthrax was present after this treatment to the extent 

 of many thousands per cubic centimetre of the liquid. 



Clearly, therefore, the anthrax is not poisoned off by the secretions 

 of B. fluorescens liquefaciens. 



These results suggested the following simple experiment : — 

 Four sets of test-tubes, properly prepared, were arranged as fol- 

 lows : — 



One set (A) received a charge of the green fluorescing liquid ob- 

 tained by allowing Bacillus fluorescens to thoroughly liquefy gelatine, 

 the charge being simply filtered at ordinary temperatures through 

 sterilised filter paper; this merely holds back the large flocculent 

 masses of the Schizomycete, and allows numerous isolated ones to 

 pass. 



Set B received an exactly similar charge, but was then put into 

 a beaker of water kept boiling for fifteen minutes. 



A third set (C) was charged with equal quantities of ordinary 

 nutrient gelatine and of the above green liquid. 



VOL. LTII. X 



